Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand

Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand

A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/...

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Main Authors: Alan Brockman, Sittaporn Singlam, Lucy Phiaphun, Sornchai Looareesuwan, Nicholas J. White, François Nosten
Other Authors: Shoklo Malaria Research Unit
Format: Article
Published: 2018
Subjects:
Medicine
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/21686
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spelling th-mahidol.216862018-07-24T10:52:56Z Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand Alan Brockman Sittaporn Singlam Lucy Phiaphun Sornchai Looareesuwan Nicholas J. White François Nosten Shoklo Malaria Research Unit Mahidol University University of Queensland John Radcliffe Hospital Menzies School of Health Research Medicine A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/μl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level. 2018-07-24T03:52:56Z 2018-07-24T03:52:56Z 2004-04-01 Article Antimicrobial Agents and Chemotherapy. Vol.48, No.4 (2004), 1426-1429 10.1128/AAC.48.4.1426-1429.2004 00664804 2-s2.0-1642543069 https://repository.li.mahidol.ac.th/handle/123456789/21686 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=1642543069&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Alan Brockman
Sittaporn Singlam
Lucy Phiaphun
Sornchai Looareesuwan
Nicholas J. White
François Nosten
Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
description A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/μl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.
author2 Shoklo Malaria Research Unit
author_facet Shoklo Malaria Research Unit
Alan Brockman
Sittaporn Singlam
Lucy Phiaphun
Sornchai Looareesuwan
Nicholas J. White
François Nosten
format Article
author Alan Brockman
Sittaporn Singlam
Lucy Phiaphun
Sornchai Looareesuwan
Nicholas J. White
François Nosten
author_sort Alan Brockman
title Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
title_short Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
title_full Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
title_fullStr Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
title_full_unstemmed Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
title_sort field evaluation of a novel colorimetric method - double-site enzyme-linked lactate dehydrogenase immunodetection assay - to determine drug susceptibilities of plasmodium falciparum clinical isolates from northwestern thailand
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/21686
_version_ 1763492334904803328

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