Direct Identification of Mycobacterium Tuberculosis from Sputum on Ziehl-Neelsen Acid Fast Stained Slides by Use of Silica-Based Filter Combined with Polymerase Chain Reaction Assay
This paper describes a method for isolation of deoxyribonucleic acid (DNA) from Ziehl-Neelsen stained sputum smears on glass slides; and isolated DNA was used for the IS6110 polymerase chain reaction (PCR)-based identification of M. tuberculosis. A total of 221 samples from newly diagnosed suspected...
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Main Authors: | , , |
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Format: | Article |
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2018
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Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/21737 |
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Institution: | Mahidol University |
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