Anti-venom potential of butanolic extract of Eclipta prostrata against Malayan pit viper venom

• Main Authors: Pimolpan Pithayanukul, Sasitorn Laovachirasuwan, Rapepol Bavovada, Narumol Pakmanee, Rutt Suttisri
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Pharmacology, Toxicology and Pharmaceutics
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/21814

The butanolic and purified butanolic extracts (PBEs) of Eclipta prostrata were evaluated for their anti-venom potential. Inhibition of lethal, hemorrhagic, proteolytic, and phospholipase A2activities of Calloselasma rhodostoma (Malayan pit viper (MPV)) venom by these extracts were determined. Demethylwedelolactone was identified as their major constituent. The butanolic extract, at 2.5mg per mouse, was able to completely neutralize the lethal activity of 2LD50of MPV venom, but increasing the dose diminished the effect. The PBE, at 1.5-4.5mg per mouse, was able to neutralize the lethality of the venom at around 50-58%. Both extracts partially inhibited the hemorrhagic activity but displayed very low anti-phospholipase A2activity and did not inhibit proteolytic activity of MPV venom. © 2003 Elsevier Ireland Ltd. All rights reserved.