Characterization of the Opisthorchis viverrini genome
The methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes Mspl, Hpall, Hhal were used to probe CpG methylation while Mbol, Sau3A, Dpnl were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola...
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| Main Authors: | , , |
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| Format: | Article |
| Published: |
2018
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| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/21972 |
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| Institution: | Mahidol University |
| Summary: | The methylations of trematode genomic DNA were analyzed using restriction enzymes and Southern blot hybridization. Restriction enzymes Mspl, Hpall, Hhal were used to probe CpG methylation while Mbol, Sau3A, Dpnl were used for A methylation. The results revealed that Opisthorchis viverrini, Fasciola gigantica and Gigantocotyle siamensis had neither CpG nor A methylations. The presence of highly repeated DNA elements was also demonstrated in O. viverrini genomic DNA. © 1991, Cambridge University Press. All rights reserved. |
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