Improved resolution and sensitivity of human DNA fingerprinting by specific-primed labelling of M13 DNA

• Main Authors: Anchalee Tassanakajon, Chainarong Wongteerasapaya, Pornpan Pumichoti, Vichai Boonsaeng, Sakol Panyim
• Other Authors: Srinakharinwirot University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/22003
• Institution: Mahidol University

A method to label M13 DNA probe by primer extension using a specific oligonucleotide primer is described. The method specifically labels the two 15-bp repeats in M13 DNA which hybridize to target DNA giving rise to DNA fingerprinting patterns. The M13 probe labelled by this method gave superior DNA...