Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula

Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula

Summary Two monoclonal antibodies have been produced that bind to the common epitopes on adult and schistosomula of S. japonicum (Chinese strain). The isotypes of these monoclonal antibodies were determined to be IgM (designated SG9‐5) and IgG2a (designed 9E7) respectively. The target epitopes of 8...

Full description

Saved in:
Bibliographic Details
Main Authors: V. VIYANANT, P. SOBHON, E. S. UPATHAM, T. KUSAMRAN
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Immunology and Microbiology
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/22047
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Mahidol University
id th-mahidol.22047
record_format dspace
spelling th-mahidol.220472018-08-10T15:31:58Z Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula V. VIYANANT P. SOBHON E. S. UPATHAM T. KUSAMRAN Mahidol University Immunology and Microbiology Summary Two monoclonal antibodies have been produced that bind to the common epitopes on adult and schistosomula of S. japonicum (Chinese strain). The isotypes of these monoclonal antibodies were determined to be IgM (designated SG9‐5) and IgG2a (designed 9E7) respectively. The target epitopes of 8G9‐5 and 9E7, as analysed by immunoblolling assay, are at M, of 64 k Da and 54 kDa respectively. Indirect immunofluorescence assay using frozen sections of adult worms, intact mechanically transformed schistosomula and 5‐day‐old lung schistosomula indicated that both epitopes are located in the tegument of both stages of parasite. These epi topes appear to be two of several major immunogenic proteins on the tegument of both young and adult worms. Copyright © 1991, Wiley Blackwell. All rights reserved 2018-08-10T08:31:58Z 2018-08-10T08:31:58Z 1991-01-01 Article Parasite Immunology. Vol.13, No.6 (1991), 695-698 10.1111/j.1365-3024.1991.tb00564.x 13653024 01419838 2-s2.0-0025996949 https://repository.li.mahidol.ac.th/handle/123456789/22047 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0025996949&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
V. VIYANANT
P. SOBHON
E. S. UPATHAM
T. KUSAMRAN
Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula
description Summary Two monoclonal antibodies have been produced that bind to the common epitopes on adult and schistosomula of S. japonicum (Chinese strain). The isotypes of these monoclonal antibodies were determined to be IgM (designated SG9‐5) and IgG2a (designed 9E7) respectively. The target epitopes of 8G9‐5 and 9E7, as analysed by immunoblolling assay, are at M, of 64 k Da and 54 kDa respectively. Indirect immunofluorescence assay using frozen sections of adult worms, intact mechanically transformed schistosomula and 5‐day‐old lung schistosomula indicated that both epitopes are located in the tegument of both stages of parasite. These epi topes appear to be two of several major immunogenic proteins on the tegument of both young and adult worms. Copyright © 1991, Wiley Blackwell. All rights reserved
author2 Mahidol University
author_facet Mahidol University
V. VIYANANT
P. SOBHON
E. S. UPATHAM
T. KUSAMRAN
format Article
author V. VIYANANT
P. SOBHON
E. S. UPATHAM
T. KUSAMRAN
author_sort V. VIYANANT
title Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula
title_short Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula
title_full Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula
title_fullStr Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula
title_full_unstemmed Schistosoma japonicum (Chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula
title_sort schistosoma japonicum (chinese strain): characterization of two monoclonal antibodies which recognized common epitopes in the tegument of adult worms and schistosomula
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/22047
_version_ 1763491284518961152

Similar Items