Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid
Undifferentiated callus and regenerating shoot callus on Murashige and Skoog (MS) medium was unable to metabolize scopolamine to hyoscyamine. Shoots on MS medium supplemented with hyoscyamine on extraction afforded unchanged hyoscyamine (44%). However, 16% was converted to 6-hydroxyhyoscyamine and 1...
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th-mahidol.222432018-08-10T15:44:19Z Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid Wandee Gritsanapan William J. Griffin Mahidol University University of Queensland Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Undifferentiated callus and regenerating shoot callus on Murashige and Skoog (MS) medium was unable to metabolize scopolamine to hyoscyamine. Shoots on MS medium supplemented with hyoscyamine on extraction afforded unchanged hyoscyamine (44%). However, 16% was converted to 6-hydroxyhyoscyamine and 12% to scopolamine. Metabolism appeared unaffected by the number of shoots formed. Callus only gave very low yields of these two metabolities. Control callus and shoots on MS medium without alkaloid contained traces of valtropine and/or butropine. However, both callus and shoots on MS medium supplemented with either scopolamine or hyoscyamine showed enhanced levels of these minor alkaloids at times by a factor exceeding 500. © 1992. 2018-08-10T08:43:59Z 2018-08-10T08:43:59Z 1992-01-01 Article Phytochemistry. Vol.31, No.9 (1992), 3069-3071 10.1016/0031-9422(92)83448-8 00319422 2-s2.0-0010539778 https://repository.li.mahidol.ac.th/handle/123456789/22243 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0010539778&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Wandee Gritsanapan William J. Griffin Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid |
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Undifferentiated callus and regenerating shoot callus on Murashige and Skoog (MS) medium was unable to metabolize scopolamine to hyoscyamine. Shoots on MS medium supplemented with hyoscyamine on extraction afforded unchanged hyoscyamine (44%). However, 16% was converted to 6-hydroxyhyoscyamine and 12% to scopolamine. Metabolism appeared unaffected by the number of shoots formed. Callus only gave very low yields of these two metabolities. Control callus and shoots on MS medium without alkaloid contained traces of valtropine and/or butropine. However, both callus and shoots on MS medium supplemented with either scopolamine or hyoscyamine showed enhanced levels of these minor alkaloids at times by a factor exceeding 500. © 1992. |
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Mahidol University |
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Mahidol University Wandee Gritsanapan William J. Griffin |
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Article |
author |
Wandee Gritsanapan William J. Griffin |
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Wandee Gritsanapan |
title |
Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid |
title_short |
Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid |
title_full |
Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid |
title_fullStr |
Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid |
title_full_unstemmed |
Alkaloid metabolism in callus and regenerating shoot cultures of a Duboisia hybrid |
title_sort |
alkaloid metabolism in callus and regenerating shoot cultures of a duboisia hybrid |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/22243 |
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1763493932156583936 |