High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity

A reversed-phase high-performance liquid chromatographic technique for the determination of dihydroorotate dehydrogenase in Plasmodium falciparum was developed. The assay was applied to the evaluation of the effects of several antimalarial drugs on the enzyme. Treatment of both the asexual and gamet...

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Main Authors:	Isra Ittarat, H. Kyle Webster, Yongyuth Yuthavong
Other Authors:	Mahidol University
Format:	Article
Published:	2018
Subjects:	Biochemistry, Genetics and Molecular Biology Chemistry
Online Access:	https://repository.li.mahidol.ac.th/handle/123456789/22252
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Institution:	Mahidol University

id	th-mahidol.22252
record_format	dspace
spelling	th-mahidol.222522018-08-10T15:44:45Z High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity Isra Ittarat H. Kyle Webster Yongyuth Yuthavong Mahidol University Armed Forces Research Institute of Medical Sciences, Thailand Biochemistry, Genetics and Molecular Biology Chemistry A reversed-phase high-performance liquid chromatographic technique for the determination of dihydroorotate dehydrogenase in Plasmodium falciparum was developed. The assay was applied to the evaluation of the effects of several antimalarial drugs on the enzyme. Treatment of both the asexual and gametocyte stages of P. falciparum in culture with menoctone, primaquine or the primaquine derivative WR 238605 led to depression of the enzyme activity, although the drugs did not appear to inhibit the enzyme directly. © 1992. 2018-08-10T08:44:10Z 2018-08-10T08:44:10Z 1992-11-06 Article Journal of Chromatography B: Biomedical Sciences and Applications. Vol.582, No.1-2 (1992), 57-64 10.1016/0378-4347(92)80302-7 03784347 2-s2.0-0026455012 https://repository.li.mahidol.ac.th/handle/123456789/22252 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0026455012&origin=inward
institution	Mahidol University
building	Mahidol University Library
continent	Asia
country	Thailand Thailand
content_provider	Mahidol University Library
collection	Mahidol University Institutional Repository
topic	Biochemistry, Genetics and Molecular Biology Chemistry
spellingShingle	Biochemistry, Genetics and Molecular Biology Chemistry Isra Ittarat H. Kyle Webster Yongyuth Yuthavong High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity
description	A reversed-phase high-performance liquid chromatographic technique for the determination of dihydroorotate dehydrogenase in Plasmodium falciparum was developed. The assay was applied to the evaluation of the effects of several antimalarial drugs on the enzyme. Treatment of both the asexual and gametocyte stages of P. falciparum in culture with menoctone, primaquine or the primaquine derivative WR 238605 led to depression of the enzyme activity, although the drugs did not appear to inhibit the enzyme directly. © 1992.
author2	Mahidol University
author_facet	Mahidol University Isra Ittarat H. Kyle Webster Yongyuth Yuthavong
format	Article
author	Isra Ittarat H. Kyle Webster Yongyuth Yuthavong
author_sort	Isra Ittarat
title	High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity
title_short	High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity
title_full	High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity
title_fullStr	High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity
title_full_unstemmed	High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity
title_sort	high-performance liquid chromatographic determination of dihydroorotate dehydrogenase of plasmodium falciparum and effects of antimalarials on enzyme activity
publishDate	2018
url	https://repository.li.mahidol.ac.th/handle/123456789/22252
_version_	1763490732310528000

High-performance liquid chromatographic determination of dihydroorotate dehydrogenase of Plasmodium falciparum and effects of antimalarials on enzyme activity

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