A specific DNA probe which identifies Babesia bovis in whole blood
A genomic library of Babesia bovis DNA from the Mexican strain M was constructed in plasmid pUN121 and cloned in Escherichia coli. Several recombinants which hybridized strongly to radioactively labeled B. bovis genomic DNA in an situ screening were selected and further analyzed for those which spec...
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th-mahidol.223052018-08-10T15:50:38Z A specific DNA probe which identifies Babesia bovis in whole blood Wasana Petchpoo Peerapan Tan-ariya Vichai Boonsaeng Chariya R. Brockelman Prapon Wilairat Sakol Panyim Srinakharinwirot University Mahidol University Immunology and Microbiology Veterinary A genomic library of Babesia bovis DNA from the Mexican strain M was constructed in plasmid pUN121 and cloned in Escherichia coli. Several recombinants which hybridized strongly to radioactively labeled B. bovis genomic DNA in an situ screening were selected and further analyzed for those which specifically hybridized to B. bovis DNA. It was found that pMU-B1 had the highest sensitivity, detecting 25 pg of purified B. bovis DNA, and 300 parasites in 10 μl of whole infected blood, or 0.00025% parasiteemia. pMU-B1 contained a 6.0 kb B. bovis DNA insert which did not cross-hybridize to Babesia bigemina, Trypanosoma evansi, Plasmodium falciparum, Anaplasma marginale, Boophilus microplus and cow DNA. In the Southern blot analysis of genomic DNA, pMU-B1 could differentiate between two B. bovis geographic isolates, Mexican strain M and Thai isolate TS4. Thus, the pMU-B1 probe will be useful in the diagnosis of Babesia infection in cattle and ticks, and in the differentiation of B. bovis strains. © 1992. 2018-08-10T08:45:19Z 2018-08-10T08:45:19Z 1992-01-01 Article Veterinary Parasitology. Vol.42, No.3-4 (1992), 189-198 10.1016/0304-4017(92)90061-D 03044017 2-s2.0-0026636008 https://repository.li.mahidol.ac.th/handle/123456789/22305 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0026636008&origin=inward |
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Immunology and Microbiology Veterinary Wasana Petchpoo Peerapan Tan-ariya Vichai Boonsaeng Chariya R. Brockelman Prapon Wilairat Sakol Panyim A specific DNA probe which identifies Babesia bovis in whole blood |
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A genomic library of Babesia bovis DNA from the Mexican strain M was constructed in plasmid pUN121 and cloned in Escherichia coli. Several recombinants which hybridized strongly to radioactively labeled B. bovis genomic DNA in an situ screening were selected and further analyzed for those which specifically hybridized to B. bovis DNA. It was found that pMU-B1 had the highest sensitivity, detecting 25 pg of purified B. bovis DNA, and 300 parasites in 10 μl of whole infected blood, or 0.00025% parasiteemia. pMU-B1 contained a 6.0 kb B. bovis DNA insert which did not cross-hybridize to Babesia bigemina, Trypanosoma evansi, Plasmodium falciparum, Anaplasma marginale, Boophilus microplus and cow DNA. In the Southern blot analysis of genomic DNA, pMU-B1 could differentiate between two B. bovis geographic isolates, Mexican strain M and Thai isolate TS4. Thus, the pMU-B1 probe will be useful in the diagnosis of Babesia infection in cattle and ticks, and in the differentiation of B. bovis strains. © 1992. |
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Srinakharinwirot University |
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Srinakharinwirot University Wasana Petchpoo Peerapan Tan-ariya Vichai Boonsaeng Chariya R. Brockelman Prapon Wilairat Sakol Panyim |
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Wasana Petchpoo Peerapan Tan-ariya Vichai Boonsaeng Chariya R. Brockelman Prapon Wilairat Sakol Panyim |
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Wasana Petchpoo |
title |
A specific DNA probe which identifies Babesia bovis in whole blood |
title_short |
A specific DNA probe which identifies Babesia bovis in whole blood |
title_full |
A specific DNA probe which identifies Babesia bovis in whole blood |
title_fullStr |
A specific DNA probe which identifies Babesia bovis in whole blood |
title_full_unstemmed |
A specific DNA probe which identifies Babesia bovis in whole blood |
title_sort |
specific dna probe which identifies babesia bovis in whole blood |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/22305 |
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1763492988521021440 |