Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode

The rhg1 gene or genes lie at a recessive or co-dominant locus, necessary for resistance to all Hg types of the soybean (Glycine max (L.) Merr.) cyst nematode (Heterodera glycines I.). The aim here was to identify nucleotide changes within a candidate gene found at the rhg1 locus that were capable o...

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Main Authors: E. Ruben, A. Jamai, J. Afzal, V. N. Njiti, K. Triwitayakorn, M. J. Iqbal, S. Yaegashi, R. Bashir, S. Kazi, P. Arelli, C. D. Town, H. Ishihara, K. Meksem, D. A. Lightfoot
Other Authors: Southern Illinois University at Carbondale
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/22944
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spelling th-mahidol.229442018-08-20T13:49:10Z Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode E. Ruben A. Jamai J. Afzal V. N. Njiti K. Triwitayakorn M. J. Iqbal S. Yaegashi R. Bashir S. Kazi P. Arelli C. D. Town H. Ishihara K. Meksem D. A. Lightfoot Southern Illinois University at Carbondale National Center for Protein Structure Analysis Dartmouth College Mahidol University Alcorn State University University of Tokyo United States Department of Agriculture J. Craig Venter Institute Universitat Bielefeld Biochemistry, Genetics and Molecular Biology The rhg1 gene or genes lie at a recessive or co-dominant locus, necessary for resistance to all Hg types of the soybean (Glycine max (L.) Merr.) cyst nematode (Heterodera glycines I.). The aim here was to identify nucleotide changes within a candidate gene found at the rhg1 locus that were capable of altering resistance to Hg types 0 (race 3). A 1.5 ± 0.25 cM region of chromosome 18 (linkage group G) was shown to encompass rhg1 using recombination events from four near isogenic line populations and nine DNA markers. The DNA markers anchored two bacterial artificial chromosome (BAC) clones 21d9 and 73p6. A single receptor like kinase (RLK; leucine rich repeat-transmembrane-protein kinase) candidate resistance gene was amplified from both BACs using redundant primers. The DNA sequence showed nine alleles of the RLK at Rhg1 in the soybean germplasm. Markers designed to detect alleles showed perfect association between allele 1 and resistance to soybean cyst nematode Hg types 0 in three segregating populations, fifteen additional selected recombination events and twenty-two Plant Introductions. A quantitative trait nucleotide in the RLK at rhg1 was inferred that alters A47 to V47 in the context of H297 rather than N297. Contiguous DNA sequence of 315 kbp of chromosome 18 (about 2 cM) contained additional gene candidates that may modulate resistance to other Hg-types including a variant laccase, a hydrogen-sodium ion antiport and two proteins of unknown function. A molecular basis for recessive and co-dominant resistance that involves interactions among paralagous disease-resistance genes was inferred that would improve methods for developing new nematode-resistant soybean cultivars. © 2006 Springer-Verlag. 2018-08-20T06:49:10Z 2018-08-20T06:49:10Z 2006-12-01 Article Molecular Genetics and Genomics. Vol.276, No.6 (2006), 503-516 10.1007/s00438-006-0150-8 16174615 2-s2.0-33749117257 https://repository.li.mahidol.ac.th/handle/123456789/22944 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33749117257&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
spellingShingle Biochemistry, Genetics and Molecular Biology
E. Ruben
A. Jamai
J. Afzal
V. N. Njiti
K. Triwitayakorn
M. J. Iqbal
S. Yaegashi
R. Bashir
S. Kazi
P. Arelli
C. D. Town
H. Ishihara
K. Meksem
D. A. Lightfoot
Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode
description The rhg1 gene or genes lie at a recessive or co-dominant locus, necessary for resistance to all Hg types of the soybean (Glycine max (L.) Merr.) cyst nematode (Heterodera glycines I.). The aim here was to identify nucleotide changes within a candidate gene found at the rhg1 locus that were capable of altering resistance to Hg types 0 (race 3). A 1.5 ± 0.25 cM region of chromosome 18 (linkage group G) was shown to encompass rhg1 using recombination events from four near isogenic line populations and nine DNA markers. The DNA markers anchored two bacterial artificial chromosome (BAC) clones 21d9 and 73p6. A single receptor like kinase (RLK; leucine rich repeat-transmembrane-protein kinase) candidate resistance gene was amplified from both BACs using redundant primers. The DNA sequence showed nine alleles of the RLK at Rhg1 in the soybean germplasm. Markers designed to detect alleles showed perfect association between allele 1 and resistance to soybean cyst nematode Hg types 0 in three segregating populations, fifteen additional selected recombination events and twenty-two Plant Introductions. A quantitative trait nucleotide in the RLK at rhg1 was inferred that alters A47 to V47 in the context of H297 rather than N297. Contiguous DNA sequence of 315 kbp of chromosome 18 (about 2 cM) contained additional gene candidates that may modulate resistance to other Hg-types including a variant laccase, a hydrogen-sodium ion antiport and two proteins of unknown function. A molecular basis for recessive and co-dominant resistance that involves interactions among paralagous disease-resistance genes was inferred that would improve methods for developing new nematode-resistant soybean cultivars. © 2006 Springer-Verlag.
author2 Southern Illinois University at Carbondale
author_facet Southern Illinois University at Carbondale
E. Ruben
A. Jamai
J. Afzal
V. N. Njiti
K. Triwitayakorn
M. J. Iqbal
S. Yaegashi
R. Bashir
S. Kazi
P. Arelli
C. D. Town
H. Ishihara
K. Meksem
D. A. Lightfoot
format Article
author E. Ruben
A. Jamai
J. Afzal
V. N. Njiti
K. Triwitayakorn
M. J. Iqbal
S. Yaegashi
R. Bashir
S. Kazi
P. Arelli
C. D. Town
H. Ishihara
K. Meksem
D. A. Lightfoot
author_sort E. Ruben
title Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode
title_short Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode
title_full Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode
title_fullStr Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode
title_full_unstemmed Genomic analysis of the rhg1 locus: Candidate genes that underlie soybean resistance to the cyst nematode
title_sort genomic analysis of the rhg1 locus: candidate genes that underlie soybean resistance to the cyst nematode
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/22944
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