Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers

• Main Authors: Panadda Boonserm, Seangduen Moonsom, Chanikarn Boonchoy, Boonhiang Promdonkoy, Krupakar Parthasarathy, Jaume Torres
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/23049

We show herein that interaction in aqueous solution of the two components of binary toxin from Bacillus sphaericus, BinA and BinB, leads to a dramatic conformational change, from β turns or random coil, to β structure. Also, either BinA or BinB separately or their equimolar mixture, interact with lipid bilayers resulting in further conformational changes. Upon membrane association, the change in conformation observed for BinA or BinB separately is different from that observed when the proteins are combined, indicating that proper folding depends on the presence of the complementary subunit. We also show, in contrast to previous reports, that BinB, but not BinA, is able to insert in model neutral lipid monolayers. © 2006 Elsevier Inc. All rights reserved.