Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers
We show herein that interaction in aqueous solution of the two components of binary toxin from Bacillus sphaericus, BinA and BinB, leads to a dramatic conformational change, from β turns or random coil, to β structure. Also, either BinA or BinB separately or their equimolar mixture, interact with li...
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th-mahidol.230492018-08-20T13:51:59Z Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers Panadda Boonserm Seangduen Moonsom Chanikarn Boonchoy Boonhiang Promdonkoy Krupakar Parthasarathy Jaume Torres Mahidol University The Institute of Science and Technology for Research and Development, Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Nanyang Technological University Biochemistry, Genetics and Molecular Biology We show herein that interaction in aqueous solution of the two components of binary toxin from Bacillus sphaericus, BinA and BinB, leads to a dramatic conformational change, from β turns or random coil, to β structure. Also, either BinA or BinB separately or their equimolar mixture, interact with lipid bilayers resulting in further conformational changes. Upon membrane association, the change in conformation observed for BinA or BinB separately is different from that observed when the proteins are combined, indicating that proper folding depends on the presence of the complementary subunit. We also show, in contrast to previous reports, that BinB, but not BinA, is able to insert in model neutral lipid monolayers. © 2006 Elsevier Inc. All rights reserved. 2018-08-20T06:51:59Z 2018-08-20T06:51:59Z 2006-04-21 Article Biochemical and Biophysical Research Communications. Vol.342, No.4 (2006), 1273-1278 10.1016/j.bbrc.2006.02.086 10902104 0006291X 2-s2.0-33644888306 https://repository.li.mahidol.ac.th/handle/123456789/23049 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33644888306&origin=inward |
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Biochemistry, Genetics and Molecular Biology Panadda Boonserm Seangduen Moonsom Chanikarn Boonchoy Boonhiang Promdonkoy Krupakar Parthasarathy Jaume Torres Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers |
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We show herein that interaction in aqueous solution of the two components of binary toxin from Bacillus sphaericus, BinA and BinB, leads to a dramatic conformational change, from β turns or random coil, to β structure. Also, either BinA or BinB separately or their equimolar mixture, interact with lipid bilayers resulting in further conformational changes. Upon membrane association, the change in conformation observed for BinA or BinB separately is different from that observed when the proteins are combined, indicating that proper folding depends on the presence of the complementary subunit. We also show, in contrast to previous reports, that BinB, but not BinA, is able to insert in model neutral lipid monolayers. © 2006 Elsevier Inc. All rights reserved. |
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Mahidol University |
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Mahidol University Panadda Boonserm Seangduen Moonsom Chanikarn Boonchoy Boonhiang Promdonkoy Krupakar Parthasarathy Jaume Torres |
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Panadda Boonserm Seangduen Moonsom Chanikarn Boonchoy Boonhiang Promdonkoy Krupakar Parthasarathy Jaume Torres |
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Panadda Boonserm |
title |
Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers |
title_short |
Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers |
title_full |
Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers |
title_fullStr |
Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers |
title_full_unstemmed |
Association of the components of the binary toxin from Bacillus sphaericus in solution and with model lipid bilayers |
title_sort |
association of the components of the binary toxin from bacillus sphaericus in solution and with model lipid bilayers |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/23049 |
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1763488994572632064 |