Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica
A cDNA encoding Fg14-3-3 protein 1 was cloned by immunoscreening of an adult-stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental antigens of the parasite. The protein has a deduced amino acid sequence of 252 residues and a calculated molecular weight of 28.7 kDa. It sho...
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th-mahidol.232742018-08-20T13:59:48Z Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica K. Chaithirayanon R. Grams S. Vichasri-Grams A. Hofmann G. Korge V. Viyanant E. S. Upatham P. Sobhon Mahidol University Thammasat University Freie Universitat Berlin Immunology and Microbiology A cDNA encoding Fg14-3-3 protein 1 was cloned by immunoscreening of an adult-stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental antigens of the parasite. The protein has a deduced amino acid sequence of 252 residues and a calculated molecular weight of 28.7 kDa. It shows sequence identity values between 57.6 and 58.1% to the human 14-3-3 beta, zeta, theta, and eta proteins and is in a phylogenetic cluster with the 14-3-3 protein 1 of Schistosoma spp. Nucleic acid analyses indicate that the Fg14-3-3 protein 1 is encoded by a single copy gene and that this gene is expressed as a transcript of 1250 nucleotides. In adult and 4-week-old parasites the gene's transcriptional and translational products were localized in the gut epithelium, parenchyma, tegument cells, and in the reproductive organs. An antiserum against recombinant Fg14-3-3 protein 1 detected a slightly smaller 14-3-3 protein in the parasite's excretion/secretion material and showed cross-reactivity with 14-3-3 proteins in extracts of other trematodes and mouse. Antibodies against Fg14-3-3 protein were detected in the sera of rabbits as early as 2 weeks after infection with metacercariae of F. gigantica and the antibody titre increased continuously over a 10-week observation period. © 2006 Cambridge University Press. 2018-08-20T06:59:48Z 2018-08-20T06:59:48Z 2006-12-01 Article Parasitology. Vol.133, No.6 (2006), 763-775 10.1017/S0031182006001119 14698161 00311820 2-s2.0-33751118485 https://repository.li.mahidol.ac.th/handle/123456789/23274 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33751118485&origin=inward |
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Immunology and Microbiology K. Chaithirayanon R. Grams S. Vichasri-Grams A. Hofmann G. Korge V. Viyanant E. S. Upatham P. Sobhon Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica |
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A cDNA encoding Fg14-3-3 protein 1 was cloned by immunoscreening of an adult-stage Fasciola gigantica cDNA library using a rabbit antiserum against tegumental antigens of the parasite. The protein has a deduced amino acid sequence of 252 residues and a calculated molecular weight of 28.7 kDa. It shows sequence identity values between 57.6 and 58.1% to the human 14-3-3 beta, zeta, theta, and eta proteins and is in a phylogenetic cluster with the 14-3-3 protein 1 of Schistosoma spp. Nucleic acid analyses indicate that the Fg14-3-3 protein 1 is encoded by a single copy gene and that this gene is expressed as a transcript of 1250 nucleotides. In adult and 4-week-old parasites the gene's transcriptional and translational products were localized in the gut epithelium, parenchyma, tegument cells, and in the reproductive organs. An antiserum against recombinant Fg14-3-3 protein 1 detected a slightly smaller 14-3-3 protein in the parasite's excretion/secretion material and showed cross-reactivity with 14-3-3 proteins in extracts of other trematodes and mouse. Antibodies against Fg14-3-3 protein were detected in the sera of rabbits as early as 2 weeks after infection with metacercariae of F. gigantica and the antibody titre increased continuously over a 10-week observation period. © 2006 Cambridge University Press. |
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Mahidol University |
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Mahidol University K. Chaithirayanon R. Grams S. Vichasri-Grams A. Hofmann G. Korge V. Viyanant E. S. Upatham P. Sobhon |
format |
Article |
author |
K. Chaithirayanon R. Grams S. Vichasri-Grams A. Hofmann G. Korge V. Viyanant E. S. Upatham P. Sobhon |
author_sort |
K. Chaithirayanon |
title |
Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica |
title_short |
Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica |
title_full |
Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica |
title_fullStr |
Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica |
title_full_unstemmed |
Molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in Fasciola gigantica |
title_sort |
molecular and immunological characterization of encoding gene and 14-3-3 protein 1 in fasciola gigantica |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/23274 |
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1763495896749703168 |