Genetic polymorphisms of major house dust mite allergens

Background: Polymorphic sequence substitutions in the major mite allergens can markedly affect immunoglobulin E binding and T cell responses, but there are few studies on environmental isolates from Dermatophagoides pteronyssinus and none for D. farinae. Objective: To determine the sequence variatio...

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Main Authors: S. Piboonpocanun, N. Malainual, O. Jirapongsananuruk, P. Vichyanond, W. R. Thomas
Other Authors: Mahidol University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/23344
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spelling th-mahidol.233442018-08-20T14:02:10Z Genetic polymorphisms of major house dust mite allergens S. Piboonpocanun N. Malainual O. Jirapongsananuruk P. Vichyanond W. R. Thomas Mahidol University University of Western Australia Institute of Molecular Biology and Genetics Immunology and Microbiology Background: Polymorphic sequence substitutions in the major mite allergens can markedly affect immunoglobulin E binding and T cell responses, but there are few studies on environmental isolates from Dermatophagoides pteronyssinus and none for D. farinae. Objective: To determine the sequence variation of the group 1 and 2 allergens from environmental D. pteronyssinus and D. farinae. Methods: RNA from each species was isolated from homes in Bangkok and the sequence of Der p 1, Der p 2, Der f 1, and Der f 2 determined from cDNA produced by high fidelity polymerase chain reactions. Results: The enlarged data set revealed preferred amino acid substitutions in residues 19, 81, and 215 of Der p 1 as well as sporadic changes. Der p 2 showed frequent variations with clusters of amino acid substitutions, but the canonical Der p 2.0101 was not found in any of 17 sequences. Der f 2 showed variants with clusters of substitutions similar to Der p 2 but in different amino acid positions and without any structural concordance. Der f 1 in contrast to the other allergens had few amino acid sequence substitutions. Conclusions: The sequence information on variants provides data important for the optimal design of allergen formulations and useful for the genetic engineering and structure-function analyses of the major allergens. © 2006 Blackwell Publishing Ltd. 2018-08-20T07:02:10Z 2018-08-20T07:02:10Z 2006-04-01 Article Clinical and Experimental Allergy. Vol.36, No.4 (2006), 510-516 10.1111/j.1365-2222.2006.02464.x 13652222 09547894 2-s2.0-33645323782 https://repository.li.mahidol.ac.th/handle/123456789/23344 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33645323782&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
S. Piboonpocanun
N. Malainual
O. Jirapongsananuruk
P. Vichyanond
W. R. Thomas
Genetic polymorphisms of major house dust mite allergens
description Background: Polymorphic sequence substitutions in the major mite allergens can markedly affect immunoglobulin E binding and T cell responses, but there are few studies on environmental isolates from Dermatophagoides pteronyssinus and none for D. farinae. Objective: To determine the sequence variation of the group 1 and 2 allergens from environmental D. pteronyssinus and D. farinae. Methods: RNA from each species was isolated from homes in Bangkok and the sequence of Der p 1, Der p 2, Der f 1, and Der f 2 determined from cDNA produced by high fidelity polymerase chain reactions. Results: The enlarged data set revealed preferred amino acid substitutions in residues 19, 81, and 215 of Der p 1 as well as sporadic changes. Der p 2 showed frequent variations with clusters of amino acid substitutions, but the canonical Der p 2.0101 was not found in any of 17 sequences. Der f 2 showed variants with clusters of substitutions similar to Der p 2 but in different amino acid positions and without any structural concordance. Der f 1 in contrast to the other allergens had few amino acid sequence substitutions. Conclusions: The sequence information on variants provides data important for the optimal design of allergen formulations and useful for the genetic engineering and structure-function analyses of the major allergens. © 2006 Blackwell Publishing Ltd.
author2 Mahidol University
author_facet Mahidol University
S. Piboonpocanun
N. Malainual
O. Jirapongsananuruk
P. Vichyanond
W. R. Thomas
format Article
author S. Piboonpocanun
N. Malainual
O. Jirapongsananuruk
P. Vichyanond
W. R. Thomas
author_sort S. Piboonpocanun
title Genetic polymorphisms of major house dust mite allergens
title_short Genetic polymorphisms of major house dust mite allergens
title_full Genetic polymorphisms of major house dust mite allergens
title_fullStr Genetic polymorphisms of major house dust mite allergens
title_full_unstemmed Genetic polymorphisms of major house dust mite allergens
title_sort genetic polymorphisms of major house dust mite allergens
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/23344
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