A simplified PCR methodology for semiquantitatively analyzing dengue viruses

• Main Authors: Supranee Upanan, Arturo Cabrera-Hernandez, Maneerat Ekkapongpisit, Duncan R. Smith
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/23407

The standard methodology for titrating dengue viruses, the plaque assay, is slow, time consuming and relatively expensive. Other methods require machinery that may not be routinely accessible to all researchers, particularly those in developing nations. We therefore sought to develop a rapid, simpli...