Rapid detection of dengue viral RNA in mosquitoes by nucleic acid-sequence based amplification (NASBA)

• Main Authors: Akanitt Jittmittraphap, Suwich Thammapalo, Nitipon Ratanasetyuth, Narong Wongba, Mammen P. Mammen, Wipawee Jampangern
• Other Authors: Department of Microbiology and Immunology
• Format: Article
• Published: 2018
• Subjects: Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/23516
• Institution: Mahidol University

RNA amplification by nucleic acid sequence-based amplification (NASBA) was used to detect serotype specific dengue viruses in artificially-infected female Aedes mosquitoes, in comparison with RT-PCR technique. NASBA could detect dengue virus serotype 2 and 4 below 0.1 PFU, which was more sensitive t...