Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus

Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus

Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant...

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Main Authors: P. Ruanjan, S. Kertbundit, M. Juříček
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Agricultural and Biological Sciences
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/23981
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spelling th-mahidol.239812018-08-24T08:37:15Z Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus P. Ruanjan S. Kertbundit M. Juříček Mahidol University Institute of Experimental Botany of the Academy of Sciences of the Czech Republic Agricultural and Biological Sciences Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant to virus infection during 3 years of testing. Further analysis of this clone revealed complex multicopy transgene insertion with aberrant copies. The suspected post transcriptional gene silencing was confirmed by siRNA detection. While the R0 generation of G2 transgenic papaya was found to be fully resistant to the infection, Papaya ringspot virus was able to break this resistance in subsequent generations by suppressing post-transcriptional gene silencing (PTGS). © 2007 Institute of Experimental Botany, ASCR. 2018-08-24T01:37:15Z 2018-08-24T01:37:15Z 2007-09-01 Article Biologia Plantarum. Vol.51, No.3 (2007), 517-520 10.1007/s10535-007-0110-0 00063134 2-s2.0-34250740840 https://repository.li.mahidol.ac.th/handle/123456789/23981 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34250740840&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
P. Ruanjan
S. Kertbundit
M. Juříček
Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus
description Transgenic papayas carrying the papaya ringspot virus coat protein gene were inoculated with Papaya ringspot virus. Infection was monitored by evaluating symptoms and by reverse transcription polymerase chain reaction (RT-PCR). Among eight tested transgenic lines, clone G2 was found highly resistant to virus infection during 3 years of testing. Further analysis of this clone revealed complex multicopy transgene insertion with aberrant copies. The suspected post transcriptional gene silencing was confirmed by siRNA detection. While the R0 generation of G2 transgenic papaya was found to be fully resistant to the infection, Papaya ringspot virus was able to break this resistance in subsequent generations by suppressing post-transcriptional gene silencing (PTGS). © 2007 Institute of Experimental Botany, ASCR.
author2 Mahidol University
author_facet Mahidol University
P. Ruanjan
S. Kertbundit
M. Juříček
format Article
author P. Ruanjan
S. Kertbundit
M. Juříček
author_sort P. Ruanjan
title Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus
title_short Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus
title_full Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus
title_fullStr Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus
title_full_unstemmed Post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus
title_sort post-transcriptional gene silencing is involved in resistance of transgenic papayas to papaya ringspot virus
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/23981
_version_ 1763491689387786240

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