Role of several key residues in the catalytic activity of sucrose isomerase from Klebsiella pneumoniae NK33-98-8

• Main Authors: Amornrat Aroonnual, Takuya Nihira, Tatsuji Seki, Watanalai Panbangred
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/24212

The gene encoding sucrose isomerase (palI NK33) was cloned from Klebsiella pneumoniae strain NK33-98-8. The gene was over-expressed in Escherichia coli BL21 (DE3) pLysS and its enzyme product (PalI NK33) was purified and characterized. PalI NK33 converts sucrose to 76.8% palatinose, 21.2% trehalulos...