Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice

Merozoite surface protein 1 (MSP1) is the major protein on the surface of the plasmodial merozoite, and its carboxy terminus, the 19-kDa fragment (MSP119), is highly conserved and effective in induction of a protective immune response against malaria parasite infection in mice and monkeys. However,...

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Main Authors: Pimmada Jeamwattanalert, Yuvadee Mahakunkijcharoen, Leera Kittigul, Pakpimol Mahannop, Sathit Pichyangkul, Chakrit Hirunpetcharat
Other Authors: Mahidol University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/24217
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spelling th-mahidol.242172018-08-24T09:07:42Z Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice Pimmada Jeamwattanalert Yuvadee Mahakunkijcharoen Leera Kittigul Pakpimol Mahannop Sathit Pichyangkul Chakrit Hirunpetcharat Mahidol University Armed Forces Research Institute of Medical Sciences, Thailand Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Medicine Merozoite surface protein 1 (MSP1) is the major protein on the surface of the plasmodial merozoite, and its carboxy terminus, the 19-kDa fragment (MSP119), is highly conserved and effective in induction of a protective immune response against malaria parasite infection in mice and monkeys. However, the duration of the immune response has not been elucidated. As such, we immunized BALB/c mice with a standard four-dose injection of recombinant Plasmodium yoelii MSP119 formulated with Montanide ISA51 and CpG oligodeoxynucleotide (ODN) and monitored the MSP119-specific antibody levels for up to 12 months. The antibody titers persisted constantly over the period of time without significant waning, in contrast to the antibody levels induced by immunization with Freund's adjuvant, where the antibody levels gradually declined to significantly lower levels 12 months after immunization. Investigation of immunoglobulin G (IgG) subclass longevity revealed that only the IgG1 antibody level (Th2 type-driven response) decreased significantly by 6 months, while the IgG2a antibody level (Th1 type-driven response) did not change over the 12 months after immunization, but the boosting effect was seen in the IgG1 antibody responses but not in the IgG2a antibody responses. After challenge infection, all immunized mice survived with negligibly patent parasitemia. These findings suggest that protective immune responses to MSP119 following immunization using oil-based Montanide ISA51 and CpG ODN as an adjuvant are very long-lasting and encourage clinical trials for malaria vaccine development. Copyright © 2007, American Society for Microbiology. All Rights Reserved. 2018-08-24T01:42:27Z 2018-08-24T01:42:27Z 2007-04-01 Article Clinical and Vaccine Immunology. Vol.14, No.4 (2007), 342-347 10.1128/CVI.00397-06 15566811 2-s2.0-34248386524 https://repository.li.mahidol.ac.th/handle/123456789/24217 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34248386524&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Medicine
Pimmada Jeamwattanalert
Yuvadee Mahakunkijcharoen
Leera Kittigul
Pakpimol Mahannop
Sathit Pichyangkul
Chakrit Hirunpetcharat
Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice
description Merozoite surface protein 1 (MSP1) is the major protein on the surface of the plasmodial merozoite, and its carboxy terminus, the 19-kDa fragment (MSP119), is highly conserved and effective in induction of a protective immune response against malaria parasite infection in mice and monkeys. However, the duration of the immune response has not been elucidated. As such, we immunized BALB/c mice with a standard four-dose injection of recombinant Plasmodium yoelii MSP119 formulated with Montanide ISA51 and CpG oligodeoxynucleotide (ODN) and monitored the MSP119-specific antibody levels for up to 12 months. The antibody titers persisted constantly over the period of time without significant waning, in contrast to the antibody levels induced by immunization with Freund's adjuvant, where the antibody levels gradually declined to significantly lower levels 12 months after immunization. Investigation of immunoglobulin G (IgG) subclass longevity revealed that only the IgG1 antibody level (Th2 type-driven response) decreased significantly by 6 months, while the IgG2a antibody level (Th1 type-driven response) did not change over the 12 months after immunization, but the boosting effect was seen in the IgG1 antibody responses but not in the IgG2a antibody responses. After challenge infection, all immunized mice survived with negligibly patent parasitemia. These findings suggest that protective immune responses to MSP119 following immunization using oil-based Montanide ISA51 and CpG ODN as an adjuvant are very long-lasting and encourage clinical trials for malaria vaccine development. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
author2 Mahidol University
author_facet Mahidol University
Pimmada Jeamwattanalert
Yuvadee Mahakunkijcharoen
Leera Kittigul
Pakpimol Mahannop
Sathit Pichyangkul
Chakrit Hirunpetcharat
format Article
author Pimmada Jeamwattanalert
Yuvadee Mahakunkijcharoen
Leera Kittigul
Pakpimol Mahannop
Sathit Pichyangkul
Chakrit Hirunpetcharat
author_sort Pimmada Jeamwattanalert
title Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice
title_short Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice
title_full Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice
title_fullStr Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice
title_full_unstemmed Long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of Plasmodium yoelii merozoite surface protein 1 in mice
title_sort long-lasting protective immune response to the 19-kilodalton carboxy-terminal fragment of plasmodium yoelii merozoite surface protein 1 in mice
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/24217
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