Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis

The accuracy of a Burkholderia pseudomallei 16s rRNA real-time PCR assay was evaluated against culture for the diagnosis of melioidosis in Thailand. A total of 846 samples were obtained from 383 patients with suspected melioidosis. One or more specimens were PCR positive for 47 of 77 patients with c...

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Main Authors: Narisara Chantratita, Vanaporn Wuthiekanun, Direk Limmathurotsakul, Aunchalee Thanwisai, Wasun Chantratita, N. P J Day, Sharon J. Peacock
Other Authors: Mahidol University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/24497
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spelling th-mahidol.244972018-08-24T08:59:03Z Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis Narisara Chantratita Vanaporn Wuthiekanun Direk Limmathurotsakul Aunchalee Thanwisai Wasun Chantratita N. P J Day Sharon J. Peacock Mahidol University Immunology and Microbiology Medicine The accuracy of a Burkholderia pseudomallei 16s rRNA real-time PCR assay was evaluated against culture for the diagnosis of melioidosis in Thailand. A total of 846 samples were obtained from 383 patients with suspected melioidosis. One or more specimens were PCR positive for 47 of 77 patients with culture-proven melioidosis (sensitivity 61.0%, 95% CI: 49.2-72.0%). PCR was negative for all 306 patients who were culture negative for B. pseudomallei (specificity 100%, 95% CI: 98.8-100%). Diagnostic sensitivity of PCR was 22.7% for patients who were culture positive for blood only, compared with 79.4% for patients who were culture positive for samples other than blood. The median (interquartile range) B. pseudomallei colony count in blood for 44 of 77 patients with positive blood cultures was 2.4 CFU/ml (0.2-13.5 CFU/ml); this may explain the low sensitivity of PCR for this specimen. The PCR assay described here is not sufficiently sensitive to replace culture in our clinical setting. Copyright © 2007 by The American Society of Tropical Medicine and Hygiene. 2018-08-24T01:51:29Z 2018-08-24T01:51:29Z 2007-11-01 Article American Journal of Tropical Medicine and Hygiene. Vol.77, No.5 (2007), 814-817 00029637 2-s2.0-42949113744 https://repository.li.mahidol.ac.th/handle/123456789/24497 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=42949113744&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Narisara Chantratita
Vanaporn Wuthiekanun
Direk Limmathurotsakul
Aunchalee Thanwisai
Wasun Chantratita
N. P J Day
Sharon J. Peacock
Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
description The accuracy of a Burkholderia pseudomallei 16s rRNA real-time PCR assay was evaluated against culture for the diagnosis of melioidosis in Thailand. A total of 846 samples were obtained from 383 patients with suspected melioidosis. One or more specimens were PCR positive for 47 of 77 patients with culture-proven melioidosis (sensitivity 61.0%, 95% CI: 49.2-72.0%). PCR was negative for all 306 patients who were culture negative for B. pseudomallei (specificity 100%, 95% CI: 98.8-100%). Diagnostic sensitivity of PCR was 22.7% for patients who were culture positive for blood only, compared with 79.4% for patients who were culture positive for samples other than blood. The median (interquartile range) B. pseudomallei colony count in blood for 44 of 77 patients with positive blood cultures was 2.4 CFU/ml (0.2-13.5 CFU/ml); this may explain the low sensitivity of PCR for this specimen. The PCR assay described here is not sufficiently sensitive to replace culture in our clinical setting. Copyright © 2007 by The American Society of Tropical Medicine and Hygiene.
author2 Mahidol University
author_facet Mahidol University
Narisara Chantratita
Vanaporn Wuthiekanun
Direk Limmathurotsakul
Aunchalee Thanwisai
Wasun Chantratita
N. P J Day
Sharon J. Peacock
format Article
author Narisara Chantratita
Vanaporn Wuthiekanun
Direk Limmathurotsakul
Aunchalee Thanwisai
Wasun Chantratita
N. P J Day
Sharon J. Peacock
author_sort Narisara Chantratita
title Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
title_short Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
title_full Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
title_fullStr Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
title_full_unstemmed Prospective clinical evaluation of the accuracy of 16S rRNA real-time PCR assay for the diagnosis of melioidosis
title_sort prospective clinical evaluation of the accuracy of 16s rrna real-time pcr assay for the diagnosis of melioidosis
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/24497
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