High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei
Sepsis is characterized by an uncontrolled inflammatory response to invading microorganisms. We describe the inflammatory mRNA profiles in whole-blood leukocytes, monocytes, and granulocytes using a multigene system for 35 inflammatory markers that included pro- and anti-inflammatory cytokines, chem...
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th-mahidol.245492018-08-24T09:05:31Z High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei W. Joost Wiersinga Mark C. Dessing Piet A. Kager Allen C. Cheng Direk Limmathurotsakul Nicholas P. Day Arjen M. Dondorp Tom Van Der Poll Sharon J. Peacock Academic Medical Centre, University of Amsterdam Menzies School of Health Research Mahidol University University of Oxford Immunology and Microbiology Medicine Sepsis is characterized by an uncontrolled inflammatory response to invading microorganisms. We describe the inflammatory mRNA profiles in whole-blood leukocytes, monocytes, and granulocytes using a multigene system for 35 inflammatory markers that included pro- and anti-inflammatory cytokines, chemokines, and signal transduction molecules in a case-control study with 34 patients with sepsis caused by the gram-negative bacterium Burkholderia pseudomalki (the pathogen causing melioidosis) and 32 healthy volunteers. Relative to healthy controls, patients with sepsis showed increased transcription of a whole array of inflammatory genes in peripheral blood leukocytes, granulocytes, and monocytes. Specific monocyte and granulocyte mRNA profiles were identified. Strong correlations were found between inflammatory mRNA expression levels in monocytes and clinical outcome. These data underline the notion that circulating leukocytes are an important source for inflammatory mediators in patients with gram-negative sepsis. Gene profiling such as was done here provides an excellent tool to obtain insight into the extent of inflammation activation in patients with severe infection. Copyright © 2007, American Society for Microbiology. All Rights Reserved. 2018-08-24T01:53:16Z 2018-08-24T01:53:16Z 2007-06-01 Article Infection and Immunity. Vol.75, No.6 (2007), 3074-3079 10.1128/IAI.01733-06 00199567 2-s2.0-34249894437 https://repository.li.mahidol.ac.th/handle/123456789/24549 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249894437&origin=inward |
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Immunology and Microbiology Medicine W. Joost Wiersinga Mark C. Dessing Piet A. Kager Allen C. Cheng Direk Limmathurotsakul Nicholas P. Day Arjen M. Dondorp Tom Van Der Poll Sharon J. Peacock High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei |
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Sepsis is characterized by an uncontrolled inflammatory response to invading microorganisms. We describe the inflammatory mRNA profiles in whole-blood leukocytes, monocytes, and granulocytes using a multigene system for 35 inflammatory markers that included pro- and anti-inflammatory cytokines, chemokines, and signal transduction molecules in a case-control study with 34 patients with sepsis caused by the gram-negative bacterium Burkholderia pseudomalki (the pathogen causing melioidosis) and 32 healthy volunteers. Relative to healthy controls, patients with sepsis showed increased transcription of a whole array of inflammatory genes in peripheral blood leukocytes, granulocytes, and monocytes. Specific monocyte and granulocyte mRNA profiles were identified. Strong correlations were found between inflammatory mRNA expression levels in monocytes and clinical outcome. These data underline the notion that circulating leukocytes are an important source for inflammatory mediators in patients with gram-negative sepsis. Gene profiling such as was done here provides an excellent tool to obtain insight into the extent of inflammation activation in patients with severe infection. Copyright © 2007, American Society for Microbiology. All Rights Reserved. |
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Academic Medical Centre, University of Amsterdam |
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Academic Medical Centre, University of Amsterdam W. Joost Wiersinga Mark C. Dessing Piet A. Kager Allen C. Cheng Direk Limmathurotsakul Nicholas P. Day Arjen M. Dondorp Tom Van Der Poll Sharon J. Peacock |
format |
Article |
author |
W. Joost Wiersinga Mark C. Dessing Piet A. Kager Allen C. Cheng Direk Limmathurotsakul Nicholas P. Day Arjen M. Dondorp Tom Van Der Poll Sharon J. Peacock |
author_sort |
W. Joost Wiersinga |
title |
High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei |
title_short |
High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei |
title_full |
High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei |
title_fullStr |
High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei |
title_full_unstemmed |
High-throughput mRNA profiling characterizes the expression of inflammatory molecules in sepsis caused by Burkholderia pseudomallei |
title_sort |
high-throughput mrna profiling characterizes the expression of inflammatory molecules in sepsis caused by burkholderia pseudomallei |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/24549 |
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1763494771341393920 |