Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines

Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines

EBV-PTLDs affect as high as 20% of SCT recipients especially those with T-cell depleted grafts while high mortality rates were also noted. Adoptive allogeneic and autologous CTLs have a therapeutic potential in this setting. However, the process of expansion of these cells is tedious and time consum...

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Main Authors: Sawang Petvises, Samart Pakakasama, Adisak Wongkajornsilp, Somtawin Sirireung, Wanpen Panthangkool, Suradej Hongeng
Other Authors: Thammasat University
Format: Article
Published: 2018
Subjects:
Medicine
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/24801
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spelling th-mahidol.248012018-08-24T09:02:53Z Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines Sawang Petvises Samart Pakakasama Adisak Wongkajornsilp Somtawin Sirireung Wanpen Panthangkool Suradej Hongeng Thammasat University Faculty of Medicine, Ramathibodi Hospital, Mahidol University Faculty of Medicine, Thammasat University Mahidol University Medicine EBV-PTLDs affect as high as 20% of SCT recipients especially those with T-cell depleted grafts while high mortality rates were also noted. Adoptive allogeneic and autologous CTLs have a therapeutic potential in this setting. However, the process of expansion of these cells is tedious and time consuming in both allogeneic and autologous CTL generation. For the allogeneic SCT, another major obstacle is unavailability of donors especially in an unrelated SCT setting. The aim of the present study was therefore to investigate the efficacy of autologous CIK cells (CD3+ CD56+) against autologous EBV-LCLs from post-SCT pediatric patients. We could demonstrate that CIK cells can be generated within two wk and did show the significant cytotoxicity against autologous EBV-LCLs. CIK cells may provide a potent tool for use in post-transplantation adoptive immunotherapy. © 2007 Blackwell Munksgaard. 2018-08-24T02:02:53Z 2018-08-24T02:02:53Z 2007-08-01 Article Pediatric Transplantation. Vol.11, No.5 (2007), 511-517 10.1111/j.1399-3046.2007.00692.x 13993046 13973142 2-s2.0-34447298673 https://repository.li.mahidol.ac.th/handle/123456789/24801 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34447298673&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Sawang Petvises
Samart Pakakasama
Adisak Wongkajornsilp
Somtawin Sirireung
Wanpen Panthangkool
Suradej Hongeng
Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines
description EBV-PTLDs affect as high as 20% of SCT recipients especially those with T-cell depleted grafts while high mortality rates were also noted. Adoptive allogeneic and autologous CTLs have a therapeutic potential in this setting. However, the process of expansion of these cells is tedious and time consuming in both allogeneic and autologous CTL generation. For the allogeneic SCT, another major obstacle is unavailability of donors especially in an unrelated SCT setting. The aim of the present study was therefore to investigate the efficacy of autologous CIK cells (CD3+ CD56+) against autologous EBV-LCLs from post-SCT pediatric patients. We could demonstrate that CIK cells can be generated within two wk and did show the significant cytotoxicity against autologous EBV-LCLs. CIK cells may provide a potent tool for use in post-transplantation adoptive immunotherapy. © 2007 Blackwell Munksgaard.
author2 Thammasat University
author_facet Thammasat University
Sawang Petvises
Samart Pakakasama
Adisak Wongkajornsilp
Somtawin Sirireung
Wanpen Panthangkool
Suradej Hongeng
format Article
author Sawang Petvises
Samart Pakakasama
Adisak Wongkajornsilp
Somtawin Sirireung
Wanpen Panthangkool
Suradej Hongeng
author_sort Sawang Petvises
title Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines
title_short Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines
title_full Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines
title_fullStr Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines
title_full_unstemmed Ex vivo generation of cytokine-induced killer cells (CD3+ CD56+) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines
title_sort ex vivo generation of cytokine-induced killer cells (cd3+ cd56+) from post-stem cell transplant pediatric patients against autologous-epstein-barr virus-transformed lymphoblastoid cell lines
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/24801
_version_ 1763497227676811264

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