Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis

Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis

Cochinin B, a novel ribosome-inactivating protein (RIP) with a molecular weight of 28 kDa, was purified from the seeds of Momordica cochinchinensis (Cucurbitaceae). The isolation procedure entailed ammonium sulfate precipitation, cation-exchange chromatography on SP Sepharose column and size-exclusi...

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Main Authors: Juthamas Chuethong, Kohei Oda, Hiroaki Sakurai, Ikuo Saiki, Wichet Leelamanit
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Pharmacology, Toxicology and Pharmaceutics
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/25111
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spelling th-mahidol.251112018-08-24T09:15:44Z Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis Juthamas Chuethong Kohei Oda Hiroaki Sakurai Ikuo Saiki Wichet Leelamanit Mahidol University Kyoto Institute of Technology University of Toyama Pharmacology, Toxicology and Pharmaceutics Cochinin B, a novel ribosome-inactivating protein (RIP) with a molecular weight of 28 kDa, was purified from the seeds of Momordica cochinchinensis (Cucurbitaceae). The isolation procedure entailed ammonium sulfate precipitation, cation-exchange chromatography on SP Sepharose column and size-exclusion chromatography on Superdex 75 column with a fast protein liquid chromatography (FPLC) system. The first twenty N-terminal amino acid residues of Cochinin B showed homology to type I RIPs from other Momordica species. The purified Cochinin B displayed a strong inhibitory activity on protein synthesis in the cell-free rabbit reticulocyte lysate system with IC50of 0.36 nM. Furthermore, it exhibited N-glycosidase activity and cytotoxicity against Vero cell line with IC50higher than 1540 nM. Interestingly, Cochinin B manifested strong anti-tumor activities on human cervical epithelial carcinoma (HeLa), human embryonic kidney (HEK293) and human small cell lung cancer (NCI-H187) cell lines with IC50of 16.9, 114 and 574 nM, respectively. © 2007 Pharmaceutical Society of Japan. 2018-08-24T02:15:44Z 2018-08-24T02:15:44Z 2007-03-01 Article Biological and Pharmaceutical Bulletin. Vol.30, No.3 (2007), 428-432 10.1248/bpb.30.428 13475215 09186158 2-s2.0-33847634392 https://repository.li.mahidol.ac.th/handle/123456789/25111 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847634392&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Pharmacology, Toxicology and Pharmaceutics
spellingShingle Pharmacology, Toxicology and Pharmaceutics
Juthamas Chuethong
Kohei Oda
Hiroaki Sakurai
Ikuo Saiki
Wichet Leelamanit
Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis
description Cochinin B, a novel ribosome-inactivating protein (RIP) with a molecular weight of 28 kDa, was purified from the seeds of Momordica cochinchinensis (Cucurbitaceae). The isolation procedure entailed ammonium sulfate precipitation, cation-exchange chromatography on SP Sepharose column and size-exclusion chromatography on Superdex 75 column with a fast protein liquid chromatography (FPLC) system. The first twenty N-terminal amino acid residues of Cochinin B showed homology to type I RIPs from other Momordica species. The purified Cochinin B displayed a strong inhibitory activity on protein synthesis in the cell-free rabbit reticulocyte lysate system with IC50of 0.36 nM. Furthermore, it exhibited N-glycosidase activity and cytotoxicity against Vero cell line with IC50higher than 1540 nM. Interestingly, Cochinin B manifested strong anti-tumor activities on human cervical epithelial carcinoma (HeLa), human embryonic kidney (HEK293) and human small cell lung cancer (NCI-H187) cell lines with IC50of 16.9, 114 and 574 nM, respectively. © 2007 Pharmaceutical Society of Japan.
author2 Mahidol University
author_facet Mahidol University
Juthamas Chuethong
Kohei Oda
Hiroaki Sakurai
Ikuo Saiki
Wichet Leelamanit
format Article
author Juthamas Chuethong
Kohei Oda
Hiroaki Sakurai
Ikuo Saiki
Wichet Leelamanit
author_sort Juthamas Chuethong
title Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis
title_short Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis
title_full Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis
title_fullStr Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis
title_full_unstemmed Cochinin B, a novel ribosome-inactivating protein from the seeds of Momordica cochinchinensis
title_sort cochinin b, a novel ribosome-inactivating protein from the seeds of momordica cochinchinensis
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/25111
_version_ 1763489255958511616

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