Differential diagnosis of schistosomiasis mekongi and trichinellosis in human

Differential diagnosis of schistosomiasis mekongi and trichinellosis in human

An indirect (plate) ELISA and, a more convenient version, a dot-blot (membrane) ELISA have been developed using haemocyanin of a mollusk, Megathura crenulata, i.e. keyhole limpet haemocyanin (KLH) and purified, specific antigen of Trichinella spiralis (APTsAg) obtained from a monoclonal antibody-aff...

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Bibliographic Details
Main Authors: Wannaporn Ittiprasert, Piyarat Butraporn, Viroj Kitikoon, Kosoom Klongkamnuankarn, Khamlien Pholsena, Viengxay Vanisaveth, Yuwaporn Sakolvaree, Manas Chongsa-Nguan, Pramuan Tapchaisri, Yuvadee Mahakunkijcharoen, Hisao Kurazono, Hideo Hayashi, Wanpen Chaicumpa
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Immunology and Microbiology
Medicine
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/25975
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Institution: Mahidol University
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Summary:An indirect (plate) ELISA and, a more convenient version, a dot-blot (membrane) ELISA have been developed using haemocyanin of a mollusk, Megathura crenulata, i.e. keyhole limpet haemocyanin (KLH) and purified, specific antigen of Trichinella spiralis (APTsAg) obtained from a monoclonal antibody-affinity column chromatography, for differential diagnosis of schistosomiasis mekongi and trichinellosis. Serum samples of patients with parasitologically confirmed trichinellosis were reactive to both antigens in both versions of ELISA while sera of patients with schistosomiasis mekongi were positive only to the KLH. Both ELISA were negative when used to test sera of normal controls and patients with gnathostomiasis, paragonimiasis and opisthorchiasis. (C) 2000 Elsevier Science Ireland Ltd.

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