Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand
Comparative DDT-susceptibility status as well as glutathione S-transferase activity and DDTase activity of Anopheles minimus (A), An. annuleris and Culex quinquefasciatus were investigated to ascertain the role of these enzymes in DDT-resistance. The standard WHO susceptibility test kits was used to...
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th-mahidol.260972018-09-07T16:15:43Z Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand L. Prapanthadara S. Koottathep N. Promtet W. Suwonkerd A. J. Ketterman P. Somboon Chiang Mai University Thailand Ministry of Public Health Mahidol University Medicine Comparative DDT-susceptibility status as well as glutathione S-transferase activity and DDTase activity of Anopheles minimus (A), An. annuleris and Culex quinquefasciatus were investigated to ascertain the role of these enzymes in DDT-resistance. The standard WHO susceptibility test kits was used to discriminate between resistant and susceptible populations. GST activity was measured in microtiter plates whereas DDTase activity was determined by HPLC quantitation of DDT metabolites. This is the first report of DDT-resistance in the Thai malaria vector, An. minimus species A. A positive correlation of DDT-resistance and DDTase activity was observed in this species as well as in the suspected vector, An. annularis. However, GST activity was not correlated to DDT-resistance in either species. Statistical analysis and scatter plots demonstrated the non-correlation between DDTase and GST activity in An. annularis. Studies in Culex quinquefasciatus revealed difference in GST/ DDTase and the relationship to DDT-resistance compared to the anopheline species. The Culex GST activity is correlated to DDTase activity. These results suggested that a positive correlation of GST and DDTase activity might be species dependent. 2018-09-07T09:15:43Z 2018-09-07T09:15:43Z 2000-12-01 Article Southeast Asian Journal of Tropical Medicine and Public Health. Vol.31, No.1 SUPPL. (2000), 111-118 01251562 2-s2.0-0034578313 https://repository.li.mahidol.ac.th/handle/123456789/26097 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034578313&origin=inward |
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Medicine L. Prapanthadara S. Koottathep N. Promtet W. Suwonkerd A. J. Ketterman P. Somboon Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand |
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Comparative DDT-susceptibility status as well as glutathione S-transferase activity and DDTase activity of Anopheles minimus (A), An. annuleris and Culex quinquefasciatus were investigated to ascertain the role of these enzymes in DDT-resistance. The standard WHO susceptibility test kits was used to discriminate between resistant and susceptible populations. GST activity was measured in microtiter plates whereas DDTase activity was determined by HPLC quantitation of DDT metabolites. This is the first report of DDT-resistance in the Thai malaria vector, An. minimus species A. A positive correlation of DDT-resistance and DDTase activity was observed in this species as well as in the suspected vector, An. annularis. However, GST activity was not correlated to DDT-resistance in either species. Statistical analysis and scatter plots demonstrated the non-correlation between DDTase and GST activity in An. annularis. Studies in Culex quinquefasciatus revealed difference in GST/ DDTase and the relationship to DDT-resistance compared to the anopheline species. The Culex GST activity is correlated to DDTase activity. These results suggested that a positive correlation of GST and DDTase activity might be species dependent. |
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Chiang Mai University |
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Chiang Mai University L. Prapanthadara S. Koottathep N. Promtet W. Suwonkerd A. J. Ketterman P. Somboon |
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Article |
author |
L. Prapanthadara S. Koottathep N. Promtet W. Suwonkerd A. J. Ketterman P. Somboon |
author_sort |
L. Prapanthadara |
title |
Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand |
title_short |
Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand |
title_full |
Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand |
title_fullStr |
Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand |
title_full_unstemmed |
Correlation of glutathione S-transferase and DDT dehydrochlorinase activities with DDT susceptibility in Anopheles and Culex mosquitos from northern Thailand |
title_sort |
correlation of glutathione s-transferase and ddt dehydrochlorinase activities with ddt susceptibility in anopheles and culex mosquitos from northern thailand |
publishDate |
2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/26097 |
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1763490601103261696 |