Purification and characterization of a β-lactamase from Haemophilus ducreyi in Eschedchia coil
A pCb plasmid encoding a β-lactamase from Haemophilus ducreyi was transferred to Escherichia coli, purified, and characterized. The β-lactamase could be isolated from a culture filtrate and further purified by ammonium sulfate and chelating Sepharose fast flow loaded with Zn2+. The purified enzyme r...
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| Main Authors: | , , |
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| Other Authors: | |
| Format: | Article |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/26483 |
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| Institution: | Mahidol University |