Somatic cell cloning in Buffalo (Bubalus bubalis): Effects of interspecies cytoplasmic recipients and activation procedures

• Main Authors: Yindee Kitiyanant, Jumnian Saikhun, Busabun Chaisalee, Kenneth L. White, Kanok Pavasuthipaisit
• Other Authors: The Institute of Science and Technology for Research and Development, Mahidol University
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/26486
• Institution: Mahidol University

Successful nuclear transfer (NT) of somatic cell nuclei from various mammalian species to enucleated bovine oocytes provides a universal cytoplast for NT in endangered or extinct species. Buffalo fetal fibroblasts were isolated from a day 40 fetus and were synchronized in presumptive G0by serum depr...