Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays

We made reporter HIV-1 DNA constructs carrying green fluorescent protein (GFP) gene and exchangeable env of subtype E. The recombinant constructs were used to produce infectious reporter viruses, which induced infected cells to emit green fluorescent light and rendered them easily detectable at sing...

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Main Authors: P. Auewarakul, V. Paungcharoen, S. Louisirirotchanakul, C. Wasi
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/26553
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spelling th-mahidol.265532018-09-07T16:45:25Z Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays P. Auewarakul V. Paungcharoen S. Louisirirotchanakul C. Wasi Mahidol University Immunology and Microbiology Medicine We made reporter HIV-1 DNA constructs carrying green fluorescent protein (GFP) gene and exchangeable env of subtype E. The recombinant constructs were used to produce infectious reporter viruses, which induced infected cells to emit green fluorescent light and rendered them easily detectable at single cell level. Because the env in this construct can be easily exchanged, viruses with different antigenic epitopes can be made. We used these reporter viruses to set up a neutralizing antibody assay based on fluorescence reduction by flow cytometric measurement. The result of this new assay correlated with the standard infectivity reduction assay using primary isolates. Because this new assay is faster and much less costly than the standard assay using a p24 endpoint and can be performed in peripheral blood mononuclear cells (PBMC), it provides a useful tool for analysis of HIV-1 immune responses. 2018-09-07T09:41:07Z 2018-09-07T09:41:07Z 2001-11-17 Article Asian Pacific Journal of Allergy and Immunology. Vol.19, No.2 (2001), 139-144 0125877X 2-s2.0-0034767704 https://repository.li.mahidol.ac.th/handle/123456789/26553 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034767704&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
P. Auewarakul
V. Paungcharoen
S. Louisirirotchanakul
C. Wasi
Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
description We made reporter HIV-1 DNA constructs carrying green fluorescent protein (GFP) gene and exchangeable env of subtype E. The recombinant constructs were used to produce infectious reporter viruses, which induced infected cells to emit green fluorescent light and rendered them easily detectable at single cell level. Because the env in this construct can be easily exchanged, viruses with different antigenic epitopes can be made. We used these reporter viruses to set up a neutralizing antibody assay based on fluorescence reduction by flow cytometric measurement. The result of this new assay correlated with the standard infectivity reduction assay using primary isolates. Because this new assay is faster and much less costly than the standard assay using a p24 endpoint and can be performed in peripheral blood mononuclear cells (PBMC), it provides a useful tool for analysis of HIV-1 immune responses.
author2 Mahidol University
author_facet Mahidol University
P. Auewarakul
V. Paungcharoen
S. Louisirirotchanakul
C. Wasi
format Article
author P. Auewarakul
V. Paungcharoen
S. Louisirirotchanakul
C. Wasi
author_sort P. Auewarakul
title Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
title_short Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
title_full Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
title_fullStr Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
title_full_unstemmed Application of HIV-1-green fluorescent protein (GFP) reporter viruses in neutralizing antibody assays
title_sort application of hiv-1-green fluorescent protein (gfp) reporter viruses in neutralizing antibody assays
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/26553
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