Ex-vivo short-term culture and developmental assessment of Plasmodium viva

• Main Authors: Kesinee Chotivanich, Kamolrat Silamut, Rachanee Udomsangpetch, Katarzyna A. Stepniewska, Sasithon Pukrittayakamee, Sornchai Looareesuwan, Nicholas J. White
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/26588

A simple reproducible method for short-term ex-vivo Plasmodium vivax culture is presented in which glucose, ascorbic acid, thiamine, hypoxanthine, and 50% human AB+serum are added to the standard P. falciparum in-vitro culture medium. Culture of freshly obtained blood samples from patients with acut...