Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand

Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand

Nine isoniazid (INH)-susceptible and 11 INH-resistant Mycobacterium tuberculosis clinical isolates were analyzed for katG codon 315 mutations by polymerase chain reaction (PCR) assay using primers MYC-32 and MYC-33, followed by restriction fragment length polymorphism. After AciI digestion of PCR pr...

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Main Authors: Pankorn Imwidthaya, Klaus Mieskes, Somsak Rienthong
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Medicine
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/26781
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spelling th-mahidol.267812018-09-07T16:48:30Z Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand Pankorn Imwidthaya Klaus Mieskes Somsak Rienthong Mahidol University Klinikum der Universitat Munchen Tuberculosis Bureau Medicine Nine isoniazid (INH)-susceptible and 11 INH-resistant Mycobacterium tuberculosis clinical isolates were analyzed for katG codon 315 mutations by polymerase chain reaction (PCR) assay using primers MYC-32 and MYC-33, followed by restriction fragment length polymorphism. After AciI digestion of PCR products, all 9 INH-susceptible isolates and 5 out of 11 (45%) INH-resistant isolates showed 0.12 kb band, which was previously reported to indicate wild type, whereas, 6 of 11 (55%) INH-resistant isolates lacked this band. 2018-09-07T09:48:30Z 2018-09-07T09:48:30Z 2001-06-01 Article Journal of the Medical Association of Thailand. Vol.84, No.6 (2001), 864-869 01252208 2-s2.0-0034792881 https://repository.li.mahidol.ac.th/handle/123456789/26781 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0034792881&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Medicine
spellingShingle Medicine
Pankorn Imwidthaya
Klaus Mieskes
Somsak Rienthong
Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand
description Nine isoniazid (INH)-susceptible and 11 INH-resistant Mycobacterium tuberculosis clinical isolates were analyzed for katG codon 315 mutations by polymerase chain reaction (PCR) assay using primers MYC-32 and MYC-33, followed by restriction fragment length polymorphism. After AciI digestion of PCR products, all 9 INH-susceptible isolates and 5 out of 11 (45%) INH-resistant isolates showed 0.12 kb band, which was previously reported to indicate wild type, whereas, 6 of 11 (55%) INH-resistant isolates lacked this band.
author2 Mahidol University
author_facet Mahidol University
Pankorn Imwidthaya
Klaus Mieskes
Somsak Rienthong
format Article
author Pankorn Imwidthaya
Klaus Mieskes
Somsak Rienthong
author_sort Pankorn Imwidthaya
title Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand
title_short Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand
title_full Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand
title_fullStr Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand
title_full_unstemmed Evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand
title_sort evaluation of katg codon 315 mutations among isoniazid sensitive and resistant mycobacterium tuberculosis isolates from thailand
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/26781
_version_ 1763491555842195456

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