Native and chimeric metal-binding lactate dehydrogenase as detection and protection tools for oxidative stress induced by fenton's reaction

• Main Authors: Teerawit Tangkosakul, Tanawut Tantimongcolwat, Chartchalerm Isarankura-Na-Ayudhya, Malin Mejare, Leif Bülow, Virapong Prachayasittikul
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology; Pharmacology, Toxicology and Pharmaceutics
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/26950

In the present study, a simple and reliable antioxidant screening technique based on lactate dehydrogenase (LDH) oxidation by Cu2+-mediated Fenton's reaction has successfully been developed. Oxidation of LDH by hydroxyl radical consequently leads to enzymatic inactivation, while addition of ant...