Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681)
The peritrophic membrane (PM) is a non-cellular tissue involved in the protection of midgut epithelium from mechanical damage and insults from pathogens. This study was carried out to determine the involvement of PM in mosquitoes after infection with dengue virus. Aedes aegypti (Diptera: Culicidae)...
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th-mahidol.269902018-09-13T13:18:03Z Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681) Supattra Suwanmanee Urai Chaisri Ladawan Wasinpiyamongkol Natthanej Luplertlop Mahidol University Agricultural and Biological Sciences The peritrophic membrane (PM) is a non-cellular tissue involved in the protection of midgut epithelium from mechanical damage and insults from pathogens. This study was carried out to determine the involvement of PM in mosquitoes after infection with dengue virus. Aedes aegypti (Diptera: Culicidae) mosquitoes were fed sucrose and human blood with and without dengue virus type 2 (D2-16681), and collected at 0.5, 1, 6, and 12 h, respectively. Specimens were prepared for examination under light and electron microscopy. The results showed that PM was produced only in the blood-fed mosquitoes. The infected blood meal induced the mosquitoes to produce PM in their midgut earlier and thicker than in mosquitoes with blood alone. The initial evidence of PM occurred at 1 h post-blood meal (PBM) as a matrix-like structure. By 6 h PBM, PM had become a layer, which persisted at 12 h. Among mosquitoes fed with blood alone, this structure was found only from 6 and 12 h PBM. Dengue virus type 2 induced different modifications of mosquito PM construction and structures, confirmed under an electron microscope. 2018-09-13T06:18:03Z 2018-09-13T06:18:03Z 2009-08-13 Article Applied Entomology and Zoology. Vol.44, No.2 (2009), 257-265 10.1303/aez.2009.257 1347605X 00036862 2-s2.0-68349118073 https://repository.li.mahidol.ac.th/handle/123456789/26990 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=68349118073&origin=inward |
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Agricultural and Biological Sciences Supattra Suwanmanee Urai Chaisri Ladawan Wasinpiyamongkol Natthanej Luplertlop Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681) |
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The peritrophic membrane (PM) is a non-cellular tissue involved in the protection of midgut epithelium from mechanical damage and insults from pathogens. This study was carried out to determine the involvement of PM in mosquitoes after infection with dengue virus. Aedes aegypti (Diptera: Culicidae) mosquitoes were fed sucrose and human blood with and without dengue virus type 2 (D2-16681), and collected at 0.5, 1, 6, and 12 h, respectively. Specimens were prepared for examination under light and electron microscopy. The results showed that PM was produced only in the blood-fed mosquitoes. The infected blood meal induced the mosquitoes to produce PM in their midgut earlier and thicker than in mosquitoes with blood alone. The initial evidence of PM occurred at 1 h post-blood meal (PBM) as a matrix-like structure. By 6 h PBM, PM had become a layer, which persisted at 12 h. Among mosquitoes fed with blood alone, this structure was found only from 6 and 12 h PBM. Dengue virus type 2 induced different modifications of mosquito PM construction and structures, confirmed under an electron microscope. |
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Mahidol University |
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Mahidol University Supattra Suwanmanee Urai Chaisri Ladawan Wasinpiyamongkol Natthanej Luplertlop |
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Supattra Suwanmanee Urai Chaisri Ladawan Wasinpiyamongkol Natthanej Luplertlop |
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Supattra Suwanmanee |
title |
Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681) |
title_short |
Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681) |
title_full |
Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681) |
title_fullStr |
Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681) |
title_full_unstemmed |
Peritrophic membrane structure of Aedes aegypti (Diptera: Culicidae) mosquitoes after infection with dengue virus type 2 (D2-16681) |
title_sort |
peritrophic membrane structure of aedes aegypti (diptera: culicidae) mosquitoes after infection with dengue virus type 2 (d2-16681) |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/26990 |
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1763492720090808320 |