Characterization and multiplex genotyping of 16 polymorphic microsatellite loci in the endangered bumble-bee bat, Craseonycteris thonglongyai (Chiroptera: Craseonycteridae)

• Main Authors: Sébastien J. Puechmaille, Piyathip Piyapan, Medhi Yokubol, Meriadeg Ar Gouilh, Khin Mie Mie, Paul J. Bates, Chutamas Satasook, Tin Nwe, Si Si Hla Bu, Iain MacKie, Emma C. Teeling
• Other Authors: University College Dublin
• Format: Article
• Published: 2018
• Subjects: Agricultural and Biological Sciences; Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/27000
• Institution: Mahidol University

The bumble-bee bat (Craseonycteris thonglongyai) is an endangered species with a limited distribution range and a suspected declining population. Of the 30 microsatellites developed and tested in C. thonglongyai, 16 were amplified in two multiplex polymerase chain reactions (PCRs). Based on genotypes of 64 individuals from two populations, expected heterozygosities, averaged 0.50 in Myanmar and 0.61 in Thailand. Only one pair of loci showed linkage disequilibrium in one population and no deviation from Hardy-Weinberg equilibrium was detected. This set of 16 microsatellites will be useful to study population structure and gene flow in C. thonglongyai. © 2008 Springer Science+Business Media B.V.