Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus
Chimeric reporter genes were generated comprising nine different promoters of the white spot syndrome virus linked to luciferase, with the aim to compare their transcriptional activities in insect cells. The promoters included the four non-structural genes DNA polymerase, ribonucleotide reductase sm...
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th-mahidol.271392018-09-13T13:40:55Z Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus Orawan Borirak Phenthana Samanphan Sirintip Dangtip Wansika Kiatpathomchai Sarawut Jitrapakdee Mahidol University Thailand National Center for Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Chimeric reporter genes were generated comprising nine different promoters of the white spot syndrome virus linked to luciferase, with the aim to compare their transcriptional activities in insect cells. The promoters included the four non-structural genes DNA polymerase, ribonucleotide reductase small subunit, ribonucleotide reductase large subunit, and thymidine-thymidylate kinase, and the five structural genes VP15, VP19, VP24, VP26, and VP28. The promoters of the non-structural but not the structural genes can function in these cells, indicating that transcription of the non-structural genes can be recognized by cellular transcriptional machineries. While the structural genes were highly expressed in natural host cells during white spot syndrome virus infection, their promoters failed to direct transcription in insect cells, suggesting that transcription of these late genes may require virally induced host factor(s). Motifs essential for transcription of the above non-structural genes were identified by transient transfection of insect cells with constructs containing a series of deletions in the 5′ terminal region and within the promoter. The minimal promoter sequences of these four genes were also capable of driving expression of the enhanced green fluorescent protein in insect cells. © 2009 Springer Science+Business Media, LLC. 2018-09-13T06:21:54Z 2018-09-13T06:21:54Z 2009-10-01 Article Virus Genes. Vol.39, No.2 (2009), 223-233 10.1007/s11262-009-0380-z 1572994X 09208569 2-s2.0-72549115014 https://repository.li.mahidol.ac.th/handle/123456789/27139 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=72549115014&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Orawan Borirak Phenthana Samanphan Sirintip Dangtip Wansika Kiatpathomchai Sarawut Jitrapakdee Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus |
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Chimeric reporter genes were generated comprising nine different promoters of the white spot syndrome virus linked to luciferase, with the aim to compare their transcriptional activities in insect cells. The promoters included the four non-structural genes DNA polymerase, ribonucleotide reductase small subunit, ribonucleotide reductase large subunit, and thymidine-thymidylate kinase, and the five structural genes VP15, VP19, VP24, VP26, and VP28. The promoters of the non-structural but not the structural genes can function in these cells, indicating that transcription of the non-structural genes can be recognized by cellular transcriptional machineries. While the structural genes were highly expressed in natural host cells during white spot syndrome virus infection, their promoters failed to direct transcription in insect cells, suggesting that transcription of these late genes may require virally induced host factor(s). Motifs essential for transcription of the above non-structural genes were identified by transient transfection of insect cells with constructs containing a series of deletions in the 5′ terminal region and within the promoter. The minimal promoter sequences of these four genes were also capable of driving expression of the enhanced green fluorescent protein in insect cells. © 2009 Springer Science+Business Media, LLC. |
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Mahidol University |
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Mahidol University Orawan Borirak Phenthana Samanphan Sirintip Dangtip Wansika Kiatpathomchai Sarawut Jitrapakdee |
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Article |
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Orawan Borirak Phenthana Samanphan Sirintip Dangtip Wansika Kiatpathomchai Sarawut Jitrapakdee |
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Orawan Borirak |
title |
Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus |
title_short |
Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus |
title_full |
Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus |
title_fullStr |
Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus |
title_full_unstemmed |
Promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus |
title_sort |
promoter motifs essential to the differential transcription of structural and non-structural genes of the white spot syndrome virus |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/27139 |
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1763489257840705536 |