Application of gelatin-coated magnetic particles for isolation of genomic DNA from bacterial cells

• Main Authors: Sorasak Intorasoot, Rujira Srirung, Amornrat Intorasoot, Surachai Ngamratanapaiboon
• Other Authors: Faculty of Associated Medical Sciences
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/27265
• Institution: Mahidol University

Gelatin-coated magnetic particles were implemented for bacterial genomic DNA isolation in this study. Based on structural differences in the cell wall, the standard strains Staphylococcus aureus and Escherichia coli were selected. The quantity, quality, and timing process for DNA extraction using gelatin-coated magnetite were compared to reference phenol-chloroform extraction and a commercially available kit. Approximately twice as much DNA was recovered with the use of coated magnetite, providing greater yields than other DNA extraction methods. In addition, the DNA quality was determined using 16S ribosomal DNA (rDNA) gene amplification by polymerase chain reaction (PCR). The described technique is rapid, simple, and a well-suited method to use with PCR for diagnosis of bacterial infections. © 2009 Elsevier Inc. All rights reserved.