Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0)

• Main Authors: Takuya Yamao, Yuki Eshita, Yuki Kihara, Tomomitsu Satho, Makoto Kuroda, Tsuyoshi Sekizuka, Miho Nishimura, Kouji Sakai, Shumpei Watanabe, Hiroomi Akashi, Yupha Rongsriyam, Narumon Komalamisra, Raweewan Srisawat, Takeshi Miyata, Akira Sakata, Masato Hosokawa, Manabu Nakashima, Nobuhiro Kashige, Fumio Miake, Shuetsu Fukushi, Mina Nakauchi, Masayuki Saijo, Ichiro Kurane, Shigeru Morikawa, Tetsuya Mizutani
• Other Authors: Fukuoka University
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/27747
• Institution: Mahidol University

In this study, we improved a method for rapid determination of viral RNA sequences (RDV) to overcome the limitations of previous versions. The RDV ver4.0 method can detect RNA sequences with at least 1,000 copies as starting material. A novel virus, which was isolated from field-collected Aedes aegypti larvae in the Phasi Charoen district of Thailand using C6/36 cells, was identified using the RDV ver4.0 protocol. The virus was named Phasi Charoen virus (PhaV). We used a high-throughput pyrosequencing approach to obtain more information about the genome sequence of PhaV. Analysis of a phylogenic tree based on amino acid sequences strongly suggested that PhaV belongs to the family Bunyaviridae. © 2008 Springer-Verlag.