Investigation of physicochemical condition to stabilize phosphatidylcholineliposome enclosing fluorescent calcein and its exploitation for analysis of pore formation with Cry1A toxins of Bacillus thuringiensis

• Main Authors: Kohsuke Haginoya, Vaijayanthi Thangavel, Ganesh N. Pandian, Kazuya Tomimoto, Yasuyuki Shitomi, Masaaki Azuma, Chanan Angsuthanasombat, Hidetaka Hori
• Other Authors: Niigata University
• Format: Article
• Published: 2018
• Subjects: Agricultural and Biological Sciences
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/28457
• Institution: Mahidol University

Liposome was prepared using phosphatidylcholine (PC), and calcein, a fluorescent chemical, was simultaneously enclosed within the liposome (PC-Lipo). The stability of PC-Lipo in its ability to retain calcein was evaluated under various conditions. PC-Lipo lost stability at pH 6 and pH 11-13, but was...