Cold exposure down-regulates zebrafish hematopoiesis
Erythropoiesis is regulated such that a sufficient number of mature erythrocytes is produced. Down-regulation of erythropoiesis causes various types of anemia. Although some anemia-related genes have been identified, there are several types of anemic disease for which the molecular mechanisms are ye...
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th-mahidol.287362018-09-24T15:46:11Z Cold exposure down-regulates zebrafish hematopoiesis Kasem Kulkeaw Tohru Ishitani Takaaki Kanemaru Suthat Fucharoen Daisuke Sugiyama Faculty of Dental Science Medical Institute of Bioregulation Kyushu University Hospital Mahidol University Biochemistry, Genetics and Molecular Biology Erythropoiesis is regulated such that a sufficient number of mature erythrocytes is produced. Down-regulation of erythropoiesis causes various types of anemia. Although some anemia-related genes have been identified, there are several types of anemic disease for which the molecular mechanisms are yet unclear, suggesting that unidentified genes in addition to the classical cytokine pathways play important roles in anemia. To address this issue, a new animal model for anemia is required. We established a reversible anemic model in zebrafish by keeping fish at 17 °C, a low water temperature. In zebrafish kidney marrow, expression of several genes encoding hematopoietic transcription factors (Runx1, scl, c-myb and GATA-2) and particularly erythropoiesis-related factors (klfd, hbaa1, ba1, GATA-1, EPO, and EPOr) was down-regulated, whereas myelopoiesis-related factors (csf1a and csf3) was up-regulated in low temperature conditions. We propose that this zebrafish model is useful to identify novel genes for hematopoiesis, particularly erythropoiesis. © 2010 Elsevier Inc. All rights reserved. 2018-09-24T08:46:11Z 2018-09-24T08:46:11Z 2010-04-16 Article Biochemical and Biophysical Research Communications. Vol.394, No.4 (2010), 859-864 10.1016/j.bbrc.2010.01.047 10902104 0006291X 2-s2.0-77950960835 https://repository.li.mahidol.ac.th/handle/123456789/28736 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77950960835&origin=inward |
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Biochemistry, Genetics and Molecular Biology Kasem Kulkeaw Tohru Ishitani Takaaki Kanemaru Suthat Fucharoen Daisuke Sugiyama Cold exposure down-regulates zebrafish hematopoiesis |
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Erythropoiesis is regulated such that a sufficient number of mature erythrocytes is produced. Down-regulation of erythropoiesis causes various types of anemia. Although some anemia-related genes have been identified, there are several types of anemic disease for which the molecular mechanisms are yet unclear, suggesting that unidentified genes in addition to the classical cytokine pathways play important roles in anemia. To address this issue, a new animal model for anemia is required. We established a reversible anemic model in zebrafish by keeping fish at 17 °C, a low water temperature. In zebrafish kidney marrow, expression of several genes encoding hematopoietic transcription factors (Runx1, scl, c-myb and GATA-2) and particularly erythropoiesis-related factors (klfd, hbaa1, ba1, GATA-1, EPO, and EPOr) was down-regulated, whereas myelopoiesis-related factors (csf1a and csf3) was up-regulated in low temperature conditions. We propose that this zebrafish model is useful to identify novel genes for hematopoiesis, particularly erythropoiesis. © 2010 Elsevier Inc. All rights reserved. |
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Faculty of Dental Science |
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Faculty of Dental Science Kasem Kulkeaw Tohru Ishitani Takaaki Kanemaru Suthat Fucharoen Daisuke Sugiyama |
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Article |
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Kasem Kulkeaw Tohru Ishitani Takaaki Kanemaru Suthat Fucharoen Daisuke Sugiyama |
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Kasem Kulkeaw |
title |
Cold exposure down-regulates zebrafish hematopoiesis |
title_short |
Cold exposure down-regulates zebrafish hematopoiesis |
title_full |
Cold exposure down-regulates zebrafish hematopoiesis |
title_fullStr |
Cold exposure down-regulates zebrafish hematopoiesis |
title_full_unstemmed |
Cold exposure down-regulates zebrafish hematopoiesis |
title_sort |
cold exposure down-regulates zebrafish hematopoiesis |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/28736 |
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1763490683456323584 |