Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7

Burkholderia pseudomallei is a facultative intracellular Gram-negative bacterium which is capable of surviving and multiplying inside macrophages. B. pseudomallei strain SRM117, a LPS mutant which lacks the O-antigenic polysaccharide moiety, is more susceptible to macrophage killing during the early...

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Main Authors: S. Tangsudjai, M. Pudla, K. Limposuwan, D. E. Woods, S. Sirisinha, P. Utaisincharoen
Other Authors: Mahidol University
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Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/29235
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spelling th-mahidol.292352018-09-24T16:06:23Z Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7 S. Tangsudjai M. Pudla K. Limposuwan D. E. Woods S. Sirisinha P. Utaisincharoen Mahidol University University of Calgary Immunology and Microbiology Burkholderia pseudomallei is a facultative intracellular Gram-negative bacterium which is capable of surviving and multiplying inside macrophages. B. pseudomallei strain SRM117, a LPS mutant which lacks the O-antigenic polysaccharide moiety, is more susceptible to macrophage killing during the early phase of infection than is its parental wild type strain (1026b). In this study, it was shown that the wild type is able to induce expression of genes downstream of the MyD88-dependent (iκbζ, il-6 and tnf-α), but not of the MyD88-independent (inos, ifn-βand irg-1), path ways in the mouse macrophage cell line RAW 264.7. In contrast, LPS mutant-infected macrophages were able to express genes downstream of both pathways. To elucidate the significance of activation of the MyD88-independent pathway in B. pseudomallei-infected macrophages, the expression of TBK1, an essential protein in the MyD88-independent pathway, was silenced prior to the infection. The results showed that silencing the tbk1 expression interferes with the gene expression profile in LPS mutant-infected macrophages and allows the bacteria to replicate intracellularly, thus suggesting that the MyD88-independent pathway plays an essential role in controlling intracellular survival of the LPS mutant. Moreover, exogenous IFNγ upregulated gene expression downstream of the MyD88-independent pathway, and interfered with intracellular survival in both wild type and tbk1-knockdown macrophages infected with either the wild type or the LPS mutant. These results suggest that gene expression downstream of the MyD88-independent pathway is essential in regulating the intracellular fate of B. pseudomallei, and that IFN-γ regulates gene expression through the TBK1-independent pathway. © 2010 The Societies and Blackwell Publishing Asia Pty Ltd. 2018-09-24T09:06:23Z 2018-09-24T09:06:23Z 2010-05-01 Article Microbiology and Immunology. Vol.54, No.5 (2010), 282-290 10.1111/j.1348-0421.2010.00205.x 13480421 03855600 2-s2.0-77951700590 https://repository.li.mahidol.ac.th/handle/123456789/29235 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77951700590&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
S. Tangsudjai
M. Pudla
K. Limposuwan
D. E. Woods
S. Sirisinha
P. Utaisincharoen
Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7
description Burkholderia pseudomallei is a facultative intracellular Gram-negative bacterium which is capable of surviving and multiplying inside macrophages. B. pseudomallei strain SRM117, a LPS mutant which lacks the O-antigenic polysaccharide moiety, is more susceptible to macrophage killing during the early phase of infection than is its parental wild type strain (1026b). In this study, it was shown that the wild type is able to induce expression of genes downstream of the MyD88-dependent (iκbζ, il-6 and tnf-α), but not of the MyD88-independent (inos, ifn-βand irg-1), path ways in the mouse macrophage cell line RAW 264.7. In contrast, LPS mutant-infected macrophages were able to express genes downstream of both pathways. To elucidate the significance of activation of the MyD88-independent pathway in B. pseudomallei-infected macrophages, the expression of TBK1, an essential protein in the MyD88-independent pathway, was silenced prior to the infection. The results showed that silencing the tbk1 expression interferes with the gene expression profile in LPS mutant-infected macrophages and allows the bacteria to replicate intracellularly, thus suggesting that the MyD88-independent pathway plays an essential role in controlling intracellular survival of the LPS mutant. Moreover, exogenous IFNγ upregulated gene expression downstream of the MyD88-independent pathway, and interfered with intracellular survival in both wild type and tbk1-knockdown macrophages infected with either the wild type or the LPS mutant. These results suggest that gene expression downstream of the MyD88-independent pathway is essential in regulating the intracellular fate of B. pseudomallei, and that IFN-γ regulates gene expression through the TBK1-independent pathway. © 2010 The Societies and Blackwell Publishing Asia Pty Ltd.
author2 Mahidol University
author_facet Mahidol University
S. Tangsudjai
M. Pudla
K. Limposuwan
D. E. Woods
S. Sirisinha
P. Utaisincharoen
format Article
author S. Tangsudjai
M. Pudla
K. Limposuwan
D. E. Woods
S. Sirisinha
P. Utaisincharoen
author_sort S. Tangsudjai
title Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7
title_short Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7
title_full Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7
title_fullStr Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7
title_full_unstemmed Involvement of the MyD88-independent pathway in controlling the intracellular fate of Burkholderia pseudomallei infection in the mouse macrophage cell line RAW 264.7
title_sort involvement of the myd88-independent pathway in controlling the intracellular fate of burkholderia pseudomallei infection in the mouse macrophage cell line raw 264.7
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/29235
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