Evaluation of sensitivity of multiplex PCR for detection of Mycobacterium tuberculosis and Pneumocystis jirovecii in clinical samples

• Main Authors: Sirirat Boondireke, Mathirut Mungthin, Peerapan Tan-ariya, Petchara Boonyongsunchai, Tawee Naaglor, Anan Wattanathum, Sompong Treewatchareekorn, Saovanee Leelayoova
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/29548

A multiplex PCR assay for the simultaneous detection of Mycobacterium tuberculosis and Pneumocystis jirovecii was developed using IS6110-based detection for M. tuberculosis and mitochondrial large-subunit (mtLSU) rRNA gene detection for P. jirovecii. Ninety-five pulmonary blinded samples were examin...