Identification of enterotoxigenic escherichia coli by colony hybridization using three enterotoxin gene probes

The applicability of examining clinical specimens with a DNA hybridization technique for genes encoding enterotoxins was examined using enterotoxigenic Escherichia coli (ETEC) that produced both heat-labile toxin (L T) and heat-stable toxin (ST) (24 isolates), ETEC that produced LT only (17 isolates...

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Bibliographic Details
Main Authors: S. L. Moseley, P. Echeverria, J. Seriwatana, C. Tirapat, W. Chaicumpa, T. Sakuldaipeara, S. Falkow
Other Authors: Stanford University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/30334
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Institution: Mahidol University
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Summary:The applicability of examining clinical specimens with a DNA hybridization technique for genes encoding enterotoxins was examined using enterotoxigenic Escherichia coli (ETEC) that produced both heat-labile toxin (L T) and heat-stable toxin (ST) (24 isolates), ETEC that produced LT only (17 isolates), and ETEC that produced ST only (22 isolates) from Thailand. ETEC was identified with the Y-l adrenal cell and suckling mouse assays. All were homologous with radio labeled fragments of DNA encoding L T or ST of porcine origin (ST-P) or of human origin (ST-H). Strains of ETEC that produced ST only from rural Thailand were homologous with the ST-H probe only, whereas strains isolated in Bangkok were homologous with the ST-H probe, the ST-P probe, or both probes. The hybridization technique detected ETEC in all stool samples of patients with diarrhea from whom ETEC was isolated and in ETEC-inoculated water containing other species of bacteria. The DNA hybridization assay is useful for characterizing and identifying environmental sources of ETEC. © 1982 by the University of Chicago.