High-performance liquid chromatography of aflatoxins in human urine

A method was developed for the extraction and determination of unconjugated aflatoxins in human urine by high-performance liquid chromatography. The analysis is based on the elimination of lipid-soluble constituents other than unconjugated aflatoxins in urine by light petroleum extraction. The uncon...

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Main Authors: Tongtavuch Anukarahanonta, Chutima Chudhabuddhi
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/30464
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spelling th-mahidol.304642018-10-12T14:34:44Z High-performance liquid chromatography of aflatoxins in human urine Tongtavuch Anukarahanonta Chutima Chudhabuddhi Mahidol University Chemistry A method was developed for the extraction and determination of unconjugated aflatoxins in human urine by high-performance liquid chromatography. The analysis is based on the elimination of lipid-soluble constituents other than unconjugated aflatoxins in urine by light petroleum extraction. The unconjugated aflatoxins were subsequently extracted from the aqueous phase with chloroform-acetone. Chromatography was performed isocratically with a silica column at 40°C. The resolved aflatoxins were detected and identified by ultraviolet and fluorometric detectors. The recoveries of aflatoxins B1and G1added prior to the extraction were 72% and 83%, respectively. This procedure is simple, sensitive and practically useful for epidemiological survey of unconjugated aflatoxins in human urine from areas with a high risk of aflatoxin consumption. © 1983. 2018-10-12T07:34:44Z 2018-10-12T07:34:44Z 1983-01-01 Article Journal of Chromatography B: Biomedical Sciences and Applications. Vol.275, No.C (1983), 387-393 10.1016/S0378-4347(00)84384-4 03784347 2-s2.0-0020619999 https://repository.li.mahidol.ac.th/handle/123456789/30464 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0020619999&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Chemistry
spellingShingle Chemistry
Tongtavuch Anukarahanonta
Chutima Chudhabuddhi
High-performance liquid chromatography of aflatoxins in human urine
description A method was developed for the extraction and determination of unconjugated aflatoxins in human urine by high-performance liquid chromatography. The analysis is based on the elimination of lipid-soluble constituents other than unconjugated aflatoxins in urine by light petroleum extraction. The unconjugated aflatoxins were subsequently extracted from the aqueous phase with chloroform-acetone. Chromatography was performed isocratically with a silica column at 40°C. The resolved aflatoxins were detected and identified by ultraviolet and fluorometric detectors. The recoveries of aflatoxins B1and G1added prior to the extraction were 72% and 83%, respectively. This procedure is simple, sensitive and practically useful for epidemiological survey of unconjugated aflatoxins in human urine from areas with a high risk of aflatoxin consumption. © 1983.
author2 Mahidol University
author_facet Mahidol University
Tongtavuch Anukarahanonta
Chutima Chudhabuddhi
format Article
author Tongtavuch Anukarahanonta
Chutima Chudhabuddhi
author_sort Tongtavuch Anukarahanonta
title High-performance liquid chromatography of aflatoxins in human urine
title_short High-performance liquid chromatography of aflatoxins in human urine
title_full High-performance liquid chromatography of aflatoxins in human urine
title_fullStr High-performance liquid chromatography of aflatoxins in human urine
title_full_unstemmed High-performance liquid chromatography of aflatoxins in human urine
title_sort high-performance liquid chromatography of aflatoxins in human urine
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/30464
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