Calcium Metabolism in Relation to Chronic Ethanol Ingestion and Estrogen Deficiency in Rat

• Main Authors: Nateetip Krishnamra, Liangchai Limlomwongse, Poonratana Soogaroon
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Engineering
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/30471

The plasma calcium concentration, urinary calcium excretion, total bone calcium content and the rate of calcium uptake and release by tibias were measured in 6 groups of rats which were kept under various treatment protocols for 8 wk. Ovariectomy led to significant hypocalcemia and a decrease in bone calcium content; the former being reversed towards normal by estrogen treatment. Chronic ethanol ingestion reduced the bone calcium content without apparent effect on plasma calcium in intact rat and further reduced (p<0.05) the bone calcium content in ovariectomized rats. The calcium uptake by the bone measured by 45Ca injected ip at zero hour was the same among the 6 groups. When 45Ca was injected ip 17 hours earlier, the disappearance of plasma 45Ca was found to be slower in ovariectomized and alcohol treated groups, indicating a higher rate of calcium movement from bone to plasma. Moreover, there was also an increase in the rate of calcium release by tibias after ovariectomy and chronic alcohol ingestion; the release was slightly greater in the condition of ovariectomy plus alcohol. The present investigation thus, provides evidence that ethanol is likely to have a direct stimulatory effect on bone resorption as well as an indirect effect through disrupting ovarian function during chronic administration. © 1983, The Japan Endocrine Society. All rights reserved.