Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions

Moringa oleifera Lam. (Moringaceae) possesses therapeutic potential for pharmaceutical product development. However, reports on chemical and biological stabilities of the bioactive components in the leaf extracts of M. oleifera are limited. This investigation aimed to evaluate the stability of bioac...

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Main Authors: Boonyadist Vongsak, Pongtip Sithisarn, Wandee Gritsanapan
Other Authors: Mahidol University
Format: Article
Published: 2018
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Online Access:https://repository.li.mahidol.ac.th/handle/123456789/31007
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spelling th-mahidol.310072018-10-19T11:30:04Z Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions Boonyadist Vongsak Pongtip Sithisarn Wandee Gritsanapan Mahidol University Agricultural and Biological Sciences Moringa oleifera Lam. (Moringaceae) possesses therapeutic potential for pharmaceutical product development. However, reports on chemical and biological stabilities of the bioactive components in the leaf extracts of M. oleifera are limited. This investigation aimed to evaluate the stability of bioactive compounds and free radical scavenging abilities of the leaf extracts stored under different conditions as established in the ASEAN guideline on drug stability study. The quantitative analysis of three major anti-oxidative constituents, crypto-chlorogenic acid, isoquercetin and astragalin, were performed using high-performance liquid chromatography. Total phenolics and total flavonoids contents, and DPPH free radical scavenging activity of the stored extracts were also investigated. After six months of storage, the bioactive contents and free radical scavenging activity of the extract kept at 25. ±. 2. °C with 60. ±. 5% relative humidity (RH) slightly decreased. About 50% decrease on the contents of active compounds and scavenging activity were found in the extract kept under accelerated condition at 40. ±. 2. °C with 75. ±. 5% RH. High temperature and humidity negatively impacted the contents of bioactive constituents and free radical scavenging activity of the extracts. Thus, the extracts and product from M. oleifera leaves should be kept in a cold place to prevent significant changes in chemical, physical and biological properties. © 2013 Elsevier B.V. 2018-10-19T04:30:04Z 2018-10-19T04:30:04Z 2013-07-05 Article Industrial Crops and Products. Vol.49, (2013), 419-421 10.1016/j.indcrop.2013.05.018 09266690 2-s2.0-84879588490 https://repository.li.mahidol.ac.th/handle/123456789/31007 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84879588490&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
Boonyadist Vongsak
Pongtip Sithisarn
Wandee Gritsanapan
Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions
description Moringa oleifera Lam. (Moringaceae) possesses therapeutic potential for pharmaceutical product development. However, reports on chemical and biological stabilities of the bioactive components in the leaf extracts of M. oleifera are limited. This investigation aimed to evaluate the stability of bioactive compounds and free radical scavenging abilities of the leaf extracts stored under different conditions as established in the ASEAN guideline on drug stability study. The quantitative analysis of three major anti-oxidative constituents, crypto-chlorogenic acid, isoquercetin and astragalin, were performed using high-performance liquid chromatography. Total phenolics and total flavonoids contents, and DPPH free radical scavenging activity of the stored extracts were also investigated. After six months of storage, the bioactive contents and free radical scavenging activity of the extract kept at 25. ±. 2. °C with 60. ±. 5% relative humidity (RH) slightly decreased. About 50% decrease on the contents of active compounds and scavenging activity were found in the extract kept under accelerated condition at 40. ±. 2. °C with 75. ±. 5% RH. High temperature and humidity negatively impacted the contents of bioactive constituents and free radical scavenging activity of the extracts. Thus, the extracts and product from M. oleifera leaves should be kept in a cold place to prevent significant changes in chemical, physical and biological properties. © 2013 Elsevier B.V.
author2 Mahidol University
author_facet Mahidol University
Boonyadist Vongsak
Pongtip Sithisarn
Wandee Gritsanapan
format Article
author Boonyadist Vongsak
Pongtip Sithisarn
Wandee Gritsanapan
author_sort Boonyadist Vongsak
title Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions
title_short Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions
title_full Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions
title_fullStr Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions
title_full_unstemmed Bioactive contents and free radical scavenging activity of Moringa oleifera leaf extract under different storage conditions
title_sort bioactive contents and free radical scavenging activity of moringa oleifera leaf extract under different storage conditions
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/31007
_version_ 1763496043959287808