Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells
Mangosteen (Garcinia mangostana L.) a tropical fruit, has been used in traditional medicine. A frequently used part of mangosteen is the pericarp, containing a high content of xanthones. α-Mangostin, one of the major xanthone derivatives, exhibits a variety of actions, including antimicrobial, antio...
Saved in:
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | Article |
Published: |
2018
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/31109 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Mahidol University |
id |
th-mahidol.31109 |
---|---|
record_format |
dspace |
spelling |
th-mahidol.311092018-10-19T12:45:51Z Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells Pimolkan Kasemwattanaroj Primchanien Moongkarndi Kovit Pattanapanyasat Supachoke Mangmool Ekkarat Rodpai Jutima Samer Julaporn Konlata Kasama Sukapirom Mahidol University Agricultural and Biological Sciences Medicine Pharmacology, Toxicology and Pharmaceutics Mangosteen (Garcinia mangostana L.) a tropical fruit, has been used in traditional medicine. A frequently used part of mangosteen is the pericarp, containing a high content of xanthones. α-Mangostin, one of the major xanthone derivatives, exhibits a variety of actions, including antimicrobial, antioxidant, cytotoxicand antitumor; however, its function on the immune system is still equivocal. This study aimed to examine the immunomodulatory activities of α-mangostin onlymphocyte lineage and cytokine production in human peripheral blood mononuclear cells (PBMCs). The cytotoxic activity of α-mangostin was measured by MTT assay. The concentration of α-mangostin at 5.55 μg/mL resulted in a 50% survival of PBMCs, which was as potent a cytotoxic activity as that of paclitaxel. After 24 h of PBMCs culture, the percentages of T cells (CD3+), B cells (CD19+) and NK cells (CD3-CD16+CD56+) were not significantly changed by treatment with 1, 2 and 4 μg/mL of α-mangostin compared with untreated-PBMCs; in addition, the percentages of these lymphocytes treated with the combination of α-mangostin (1, 2 and 4 μg/mL) and the mitogen concanavalin A (ConA) was not significantly different from that of ConA-treated PBMCs. For cytokine secretion, α-mangostin (1, 2 and 4 μg/mL) did not significantly induce either proinflammatory cytokines (i.e., TNF-α and IL-1β) or cytokine of adaptive immunity (i.e., IL-2). The combination of α-mangostin (1, 2 and 4 μg/mL) and ConA did not significantly alter the relative difference of TNF-α and IL-1β compared with ConA-treated PBMCs; however, these combinations could significantly decrease the relative difference of IL-2 compared with ConA-treated PBMCs. These data indicated that α-mangostin was able to inhibit IL-2 release without interfering with human immune cells; therefore, further studies are necessary to investigate its effect on IL-2 production. 2018-10-19T04:32:48Z 2018-10-19T04:32:48Z 2013-01-01 Article Natural Product Communications. Vol.8, No.9 (2013), 1257-1260 15559475 1934578X 2-s2.0-84886811015 https://repository.li.mahidol.ac.th/handle/123456789/31109 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84886811015&origin=inward |
institution |
Mahidol University |
building |
Mahidol University Library |
continent |
Asia |
country |
Thailand Thailand |
content_provider |
Mahidol University Library |
collection |
Mahidol University Institutional Repository |
topic |
Agricultural and Biological Sciences Medicine Pharmacology, Toxicology and Pharmaceutics |
spellingShingle |
Agricultural and Biological Sciences Medicine Pharmacology, Toxicology and Pharmaceutics Pimolkan Kasemwattanaroj Primchanien Moongkarndi Kovit Pattanapanyasat Supachoke Mangmool Ekkarat Rodpai Jutima Samer Julaporn Konlata Kasama Sukapirom Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells |
description |
Mangosteen (Garcinia mangostana L.) a tropical fruit, has been used in traditional medicine. A frequently used part of mangosteen is the pericarp, containing a high content of xanthones. α-Mangostin, one of the major xanthone derivatives, exhibits a variety of actions, including antimicrobial, antioxidant, cytotoxicand antitumor; however, its function on the immune system is still equivocal. This study aimed to examine the immunomodulatory activities of α-mangostin onlymphocyte lineage and cytokine production in human peripheral blood mononuclear cells (PBMCs). The cytotoxic activity of α-mangostin was measured by MTT assay. The concentration of α-mangostin at 5.55 μg/mL resulted in a 50% survival of PBMCs, which was as potent a cytotoxic activity as that of paclitaxel. After 24 h of PBMCs culture, the percentages of T cells (CD3+), B cells (CD19+) and NK cells (CD3-CD16+CD56+) were not significantly changed by treatment with 1, 2 and 4 μg/mL of α-mangostin compared with untreated-PBMCs; in addition, the percentages of these lymphocytes treated with the combination of α-mangostin (1, 2 and 4 μg/mL) and the mitogen concanavalin A (ConA) was not significantly different from that of ConA-treated PBMCs. For cytokine secretion, α-mangostin (1, 2 and 4 μg/mL) did not significantly induce either proinflammatory cytokines (i.e., TNF-α and IL-1β) or cytokine of adaptive immunity (i.e., IL-2). The combination of α-mangostin (1, 2 and 4 μg/mL) and ConA did not significantly alter the relative difference of TNF-α and IL-1β compared with ConA-treated PBMCs; however, these combinations could significantly decrease the relative difference of IL-2 compared with ConA-treated PBMCs. These data indicated that α-mangostin was able to inhibit IL-2 release without interfering with human immune cells; therefore, further studies are necessary to investigate its effect on IL-2 production. |
author2 |
Mahidol University |
author_facet |
Mahidol University Pimolkan Kasemwattanaroj Primchanien Moongkarndi Kovit Pattanapanyasat Supachoke Mangmool Ekkarat Rodpai Jutima Samer Julaporn Konlata Kasama Sukapirom |
format |
Article |
author |
Pimolkan Kasemwattanaroj Primchanien Moongkarndi Kovit Pattanapanyasat Supachoke Mangmool Ekkarat Rodpai Jutima Samer Julaporn Konlata Kasama Sukapirom |
author_sort |
Pimolkan Kasemwattanaroj |
title |
Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells |
title_short |
Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells |
title_full |
Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells |
title_fullStr |
Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells |
title_full_unstemmed |
Immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells |
title_sort |
immunomodulatory activities of α-mangostin on peripheral blood mononuclear cells |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/31109 |
_version_ |
1763488715724816384 |