Automatic pH changing system for sensitivity improvement of ELISA on lab-on-paper
In this work, automatic pi I changing system on lab-on-paper devices for enzyme-linked immunosorbent (ELISA) has been developed in order to improve their sensitivity and make the technique much more applicable to real samples. The most widely used enzyme in immunoassay are alkaline phosphatase (ALP)...
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| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Conference or Workshop Item |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/31492 |
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| Institution: | Mahidol University |
| Summary: | In this work, automatic pi I changing system on lab-on-paper devices for enzyme-linked immunosorbent (ELISA) has been developed in order to improve their sensitivity and make the technique much more applicable to real samples. The most widely used enzyme in immunoassay are alkaline phosphatase (ALP) and peroxidase. ALP-conjugated secondary detection with BCIP/NBT substrate provides high sensitivity, however, the enzymatic reaction require alkaline medium. By automatic pH changing system, the immunoreaction is allowed to undergo first in the test zone at the neutral pH. Subsequently, enzymatic reaction occurs at the alkaline pH due to switching of pH from neutral to alkaline. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved. All rights reserved. |
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