Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy
Background: The genetic population structure of Aedes (Stegomyia) aegypti (L.), the main vector of dengue virus, is being investigated in areas where a novel dengue suppression program is to be implemented. The aim of the program is to release and establish mosquito populations with impaired virus t...
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th-mahidol.326732018-10-19T12:45:52Z Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy Phanthip Olanratmanee Pattamaporn Kittayapong Chitti Chansang Ary A. Hoffmann Andrew R. Weeks Nancy M. Endersby Mahidol University National Institutes of Health, Bethesda University of Melbourne Medicine Pharmacology, Toxicology and Pharmaceutics Background: The genetic population structure of Aedes (Stegomyia) aegypti (L.), the main vector of dengue virus, is being investigated in areas where a novel dengue suppression program is to be implemented. The aim of the program is to release and establish mosquito populations with impaired virus transmission capabilities. To model effects of the release and devise protocols for its implementation, information about the genetic structure of populations at a range of spatial scales is required. Methodology/Principal Findings: This study investigates a potential release site in the Hua Sam Rong Subdistrict of Plaeng Yao District, Chachoengsao Province, in eastern Thailand which comprises a complex of five villages within a 10 km radius. Aedes aegypti resting indoors was sampled at four different times of year from houses within the five villages. Genetic markers were used to screen the mosquitoes: two Exon Primed Intron Crossing (EPIC) markers and five microsatellite markers. The raw allele size was determined using several statistical software packages to analyze the population structure of the mosquito. Estimates of effective population size for each village were low, but there was no evidence of genetic isolation by geographic distance. Conclusions: The presence of temporary genetic structure is possibly caused by genetic drift due to large contributions of adults from a few breeding containers. This suggests that the introduction of mosquitoes into an area needs to proceed through multiple releases and targeting of sites where mosquitoes are emerging in large numbers. © 2013 Olanratmanee et al. 2018-10-19T05:38:39Z 2018-10-19T05:38:39Z 2013-01-01 Article PLoS Neglected Tropical Diseases. Vol.7, No.1 (2013) 10.1371/journal.pntd.0001913 19352735 19352727 2-s2.0-84873498749 https://repository.li.mahidol.ac.th/handle/123456789/32673 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84873498749&origin=inward |
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Medicine Pharmacology, Toxicology and Pharmaceutics Phanthip Olanratmanee Pattamaporn Kittayapong Chitti Chansang Ary A. Hoffmann Andrew R. Weeks Nancy M. Endersby Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy |
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Background: The genetic population structure of Aedes (Stegomyia) aegypti (L.), the main vector of dengue virus, is being investigated in areas where a novel dengue suppression program is to be implemented. The aim of the program is to release and establish mosquito populations with impaired virus transmission capabilities. To model effects of the release and devise protocols for its implementation, information about the genetic structure of populations at a range of spatial scales is required. Methodology/Principal Findings: This study investigates a potential release site in the Hua Sam Rong Subdistrict of Plaeng Yao District, Chachoengsao Province, in eastern Thailand which comprises a complex of five villages within a 10 km radius. Aedes aegypti resting indoors was sampled at four different times of year from houses within the five villages. Genetic markers were used to screen the mosquitoes: two Exon Primed Intron Crossing (EPIC) markers and five microsatellite markers. The raw allele size was determined using several statistical software packages to analyze the population structure of the mosquito. Estimates of effective population size for each village were low, but there was no evidence of genetic isolation by geographic distance. Conclusions: The presence of temporary genetic structure is possibly caused by genetic drift due to large contributions of adults from a few breeding containers. This suggests that the introduction of mosquitoes into an area needs to proceed through multiple releases and targeting of sites where mosquitoes are emerging in large numbers. © 2013 Olanratmanee et al. |
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Mahidol University |
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Mahidol University Phanthip Olanratmanee Pattamaporn Kittayapong Chitti Chansang Ary A. Hoffmann Andrew R. Weeks Nancy M. Endersby |
format |
Article |
author |
Phanthip Olanratmanee Pattamaporn Kittayapong Chitti Chansang Ary A. Hoffmann Andrew R. Weeks Nancy M. Endersby |
author_sort |
Phanthip Olanratmanee |
title |
Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy |
title_short |
Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy |
title_full |
Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy |
title_fullStr |
Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy |
title_full_unstemmed |
Population Genetic Structure of Aedes (Stegomyia) aegypti (L.) at a Micro-Spatial Scale in Thailand: Implications for a Dengue Suppression Strategy |
title_sort |
population genetic structure of aedes (stegomyia) aegypti (l.) at a micro-spatial scale in thailand: implications for a dengue suppression strategy |
publishDate |
2018 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/32673 |
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1763489937270767616 |