Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat

The multiplex PCR (mPCR), enzyme-linked immunosorbent assay (ELISA) and immunomagnetic separation enzyme-linked immunosorbent assay (IMS-ELISA) were developed for the detection of Salmonella spp. and two specific serovars S. Typhimurium and S. Enteritidis. The detection limits of the mPCR without th...

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Main Authors: Ekkarat Rodpai, Primchanien Moongkarndi, Walla Tungrugsasut, Rangsichon Phisannoradej, Sasitorn Kanarat
Other Authors: Mahidol University
Format: Article
Published: 2018
Subjects:
Multidisciplinary
Online Access:https://repository.li.mahidol.ac.th/handle/123456789/32824
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spelling th-mahidol.328242018-10-19T12:51:01Z Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat Ekkarat Rodpai Primchanien Moongkarndi Walla Tungrugsasut Rangsichon Phisannoradej Sasitorn Kanarat Mahidol University Thailand Ministry of Agriculture and Cooperatives Multidisciplinary The multiplex PCR (mPCR), enzyme-linked immunosorbent assay (ELISA) and immunomagnetic separation enzyme-linked immunosorbent assay (IMS-ELISA) were developed for the detection of Salmonella spp. and two specific serovars S. Typhimurium and S. Enteritidis. The detection limits of the mPCR without the pre-enriched step was at 105 cfu/ml, whereas developed immunoassays demonstrated positive results at 105, 106, and 107 cfu/ml by ELISA and at 104, 104, and 106 cfu/ml by IMS-ELISA for detecting Salmonella spp., S. Typhimurium, and S. Enteritidis, respectively. The mPCR did not produce any nonspecific amplified products when tested against other related species of bacteria. In this study, the developed IMS-ELISA gave more sensitivity but less specificity than the mPCR assay. The methods are useful for the rapid detection of salmonellae in naturally infected chicken meat in Thailand. 2018-10-19T05:51:01Z 2018-10-19T05:51:01Z 2013-06-01 Article ScienceAsia. Vol.39, No.2 (2013), 150-159 10.2306/scienceasia1513-1874.2013.39.150 15131874 2-s2.0-84879366236 https://repository.li.mahidol.ac.th/handle/123456789/32824 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84879366236&origin=inward
institution Mahidol University
building Mahidol University Library
continent Asia
country Thailand
Thailand
content_provider Mahidol University Library
collection Mahidol University Institutional Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Ekkarat Rodpai
Primchanien Moongkarndi
Walla Tungrugsasut
Rangsichon Phisannoradej
Sasitorn Kanarat
Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat
description The multiplex PCR (mPCR), enzyme-linked immunosorbent assay (ELISA) and immunomagnetic separation enzyme-linked immunosorbent assay (IMS-ELISA) were developed for the detection of Salmonella spp. and two specific serovars S. Typhimurium and S. Enteritidis. The detection limits of the mPCR without the pre-enriched step was at 105 cfu/ml, whereas developed immunoassays demonstrated positive results at 105, 106, and 107 cfu/ml by ELISA and at 104, 104, and 106 cfu/ml by IMS-ELISA for detecting Salmonella spp., S. Typhimurium, and S. Enteritidis, respectively. The mPCR did not produce any nonspecific amplified products when tested against other related species of bacteria. In this study, the developed IMS-ELISA gave more sensitivity but less specificity than the mPCR assay. The methods are useful for the rapid detection of salmonellae in naturally infected chicken meat in Thailand.
author2 Mahidol University
author_facet Mahidol University
Ekkarat Rodpai
Primchanien Moongkarndi
Walla Tungrugsasut
Rangsichon Phisannoradej
Sasitorn Kanarat
format Article
author Ekkarat Rodpai
Primchanien Moongkarndi
Walla Tungrugsasut
Rangsichon Phisannoradej
Sasitorn Kanarat
author_sort Ekkarat Rodpai
title Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat
title_short Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat
title_full Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat
title_fullStr Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat
title_full_unstemmed Comparison of multiplex polymerase chain reaction and immunoassay to detect Salmonella spp., S. Typhimurium, and S. Enteritidis in Thai chicken meat
title_sort comparison of multiplex polymerase chain reaction and immunoassay to detect salmonella spp., s. typhimurium, and s. enteritidis in thai chicken meat
publishDate 2018
url https://repository.li.mahidol.ac.th/handle/123456789/32824
_version_ 1763491100134211584

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