Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns
In order to better understand the relationship between Salmonella serotypes Typhimurium and its monophasic variant 4,5,12:i:- found in Thailand, a total of 138 isolates from various sources were characterized using different molecular subtyping methods (i.e., pulsed-field gel electrophoresis [PFGE]...
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th-mahidol.330012018-11-09T09:35:30Z Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns Laingshun Huoy Srirat Pornruangwong Chaiwat Pulsrikarn Soraya Chaturongakul Mahidol University Thailand Ministry of Public Health Thailand Ministry of Education Agricultural and Biological Sciences Immunology and Microbiology Medicine In order to better understand the relationship between Salmonella serotypes Typhimurium and its monophasic variant 4,5,12:i:- found in Thailand, a total of 138 isolates from various sources were characterized using different molecular subtyping methods (i.e., pulsed-field gel electrophoresis [PFGE] and polymerase chain reaction [PCR]) and antibiotic resistance (AbR) patterns. PFGE revealed 52 distinct band patterns among these isolates, 3 of which were shared between the two serotypes. PCR characterization of genomic deletion patterns reveals that Thai S. 4,5,12:i:- isolates contain a distinct deletion pattern in the fljAB region, which can be used as a specific genetic marker for primary identification of S. 4,5,12:i:- sources. AbR study shows that, among 50 representative serotype-confirmed strains, 48.28% (14/29) of Salmonella Typhimurium and 90.48% (19/21) of 4,5,12:i:- isolates are multidrug-resistant Salmonella as they are resistant to at least 3 antimicrobial categories. The AmpST pattern for resistance to ampicillin, streptomycin, and tetracycline was found in high proportions of Salmonella Typhimurium (10 of 29) and S. 4,5,12:i:- (15 of 21) isolates. © Copyright 2014, Mary Ann Liebert, Inc. 2014. 2018-11-09T01:44:14Z 2018-11-09T01:44:14Z 2014-08-01 Article Foodborne Pathogens and Disease. Vol.11, No.8 (2014), 589-592 10.1089/fpd.2013.1723 15567125 15353141 2-s2.0-84905215782 https://repository.li.mahidol.ac.th/handle/123456789/33001 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84905215782&origin=inward |
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Agricultural and Biological Sciences Immunology and Microbiology Medicine Laingshun Huoy Srirat Pornruangwong Chaiwat Pulsrikarn Soraya Chaturongakul Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns |
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In order to better understand the relationship between Salmonella serotypes Typhimurium and its monophasic variant 4,5,12:i:- found in Thailand, a total of 138 isolates from various sources were characterized using different molecular subtyping methods (i.e., pulsed-field gel electrophoresis [PFGE] and polymerase chain reaction [PCR]) and antibiotic resistance (AbR) patterns. PFGE revealed 52 distinct band patterns among these isolates, 3 of which were shared between the two serotypes. PCR characterization of genomic deletion patterns reveals that Thai S. 4,5,12:i:- isolates contain a distinct deletion pattern in the fljAB region, which can be used as a specific genetic marker for primary identification of S. 4,5,12:i:- sources. AbR study shows that, among 50 representative serotype-confirmed strains, 48.28% (14/29) of Salmonella Typhimurium and 90.48% (19/21) of 4,5,12:i:- isolates are multidrug-resistant Salmonella as they are resistant to at least 3 antimicrobial categories. The AmpST pattern for resistance to ampicillin, streptomycin, and tetracycline was found in high proportions of Salmonella Typhimurium (10 of 29) and S. 4,5,12:i:- (15 of 21) isolates. © Copyright 2014, Mary Ann Liebert, Inc. 2014. |
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Mahidol University |
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Mahidol University Laingshun Huoy Srirat Pornruangwong Chaiwat Pulsrikarn Soraya Chaturongakul |
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Laingshun Huoy Srirat Pornruangwong Chaiwat Pulsrikarn Soraya Chaturongakul |
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Laingshun Huoy |
title |
Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns |
title_short |
Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns |
title_full |
Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns |
title_fullStr |
Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns |
title_full_unstemmed |
Molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- Reveals distinct genetic deletion patterns |
title_sort |
molecular characterization of thai salmonella enterica serotype typhimurium and serotype 4,5,12:i:- reveals distinct genetic deletion patterns |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/33001 |
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1763496001448968192 |