Forces and dynamics of glucose and inhibitor binding to sodium glucose co-transporter SGLT1 studied by single molecule force spectroscopy

• Main Authors: Isabel Neundlinger, Theeraporn Puntheeranurak, Linda Wildling, Christian Rankl, Lai Xi Wang, Hermann J. Gruber, Rolf K.H. Kinne, Peter Hinterdorfer
• Other Authors: Johannes Kepler Universitat Linz
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/33237
• Institution: Mahidol University

Single molecule force spectroscopy was employed to investigate the dynamics of the sodium glucose co-transporter (SGLT1) upon substrate and inhibitor binding on the single molecule level. CHO cells stably expressing rbSGLT1 were probed by using atomic force microscopy tips carrying either thioglucos...