Brain stem slice conditioned medium contains endogenous BDNF and GDNF that affect neural crest boundary cap cells in co-culture

• Main Authors: Andreas Kaiser, Ajay Kale, Ekaterina Novozhilova, Piyaporn Siratirakun, Jorge B. Aquino, Charoensri Thonabulsombat, Patrik Ernfors, Petri Olivius
• Other Authors: Karolinska University Hospital
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Medicine; Neuroscience
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/33259
• Institution: Mahidol University

Conditioned medium (CM), made by collecting medium after a few days in cell culture and then re-using it to further stimulate other cells, is a known experimental concept since the 1950s. Our group has explored this technique to stimulate the performance of cells in culture in general, and to evalua...